Part 1 of the experiment investigated the effect of different temperatures on beetroot cell membranes (a type of plant cell). Through this experiment, the process of diffusion and osmosis was in action. Various temperatures ranging from low temperatures to high temperatures such as -5⁰C, 5⁰C, 30⁰C, 50⁰C and 80⁰C were used to investigate the temperature effects on beetroot cell membranes.
The hypothesis predicted that the higher the temperature the darker the beetroot substance and the lower the temperature the least colour intensity represented. However this was not the case in the beetroot cell membrane experiment. A star scale was used to show the colour intensity (Key: 1/5 stars lightest colour intensity; 5/5 stars darkest colour intensity). Results showed that the highest temperature which in this case was 80⁰C, was unfortunately scaled at 1/5 stars the lightest colour intensity, a result which was definitely not expected. When analysing the rest of the results, more unexpected results appeared, showing no relation to the hypothesis prediction. Temperatures 50⁰C and 30⁰C was also scaled at 1/5 stars in colour intensity. Temperatures -5⁰C and 5⁰C were both stored in cold temperatures, -5⁰C was stored in a freezer while the 5⁰C was stored in the refrigerator. Based on the hypothesis prediction, temperature -5⁰C contained a colder temperature the 5⁰C, therefore the -5⁰C had to result in a more lighter colour intensity, as a result the 5⁰C would need to contain a darker colour intensity than -5⁰C because the 5⁰C was stored in a refrigerator, containing a temperature that is not extremely cold such as the freezer.
Information from research states that the beetroot cell membrane contains a large central vacuole containing a red pigment anthocyanin, which gives the beetroot its colour. When the beetroot cell has been disturbed or stressed such as cutting or in the case of this investigation, increasing the temperature, the beetroot’s inner vacuole