Membrane Behaviour Lab
Membrane Behavior Lab
Abstract:
Introduction:
The permeability of a cell to solutes in an aqueous medium depends upon the physical and chemical make–up of the membrane. The maintenance of the living cell depends upon the continued presence and functioning of a selectively permeable membrane. If the nature of the membrane is changed or altered in any way, this may well affect its permeability and thus the properties of the cell of which it is a part. Irreversible changes in the permeability of the membrane usually lead to the death of the cell. In this experiment, you will study the effect of changes in environmental conditions on the permeability of living beetroot cells.
Materials and Equipment:
Scalpel
Tweezers
Safety Goggles
Cork Borer
3-4 Beetroots
Small Beaker
Test tube Rack
7 Test Tubes (The Effect of Heat)
Hot Plate
10ml of 0.1M HCl
10ml of 0.01 M HCl
10ml of 0.001 M HCl
10ml of 0.001 M NaCl
10ml 0f 0.01 M NaCl
10ml of 0.1 M NaCl
10ml of tap water
9 Test Tubes (The Effect of pH)
Graduated Cylinder
6 Discs of Frozen Beetroots (3 per experiment)
10 ml of Acetone per experiment
Procedure:
The Effect of pH
1. Top and bottom of beetroot were cut off by scalpel. The cork borer was used to cut out about 3-4 cores, then each core was cut into uniform discs about 2mm thick.
2. Discs were rinsed in a small beaker of cool water for about 5 minutes.
3. Each stock solution of HCl, NaCl and tap water were measured in a graduated cylinder to 10ml, then poured into individually labeled test tubes.
4. Three beetroot discs were placed in each test tube with tweezers.
5. After 40 minute of waiting the colour and intensity was recorded.
The Effect of Freezing
1. 10 ml of room temperature water was measured with a graduated cylinder and poured into a labeled test tube.
2. Three frozen discs of beetroot were placed into the test tube of room temperature water.
3. After 40 minutes of waiting the colour and intensity
Abstract:
Introduction:
The permeability of a cell to solutes in an aqueous medium depends upon the physical and chemical make–up of the membrane. The maintenance of the living cell depends upon the continued presence and functioning of a selectively permeable membrane. If the nature of the membrane is changed or altered in any way, this may well affect its permeability and thus the properties of the cell of which it is a part. Irreversible changes in the permeability of the membrane usually lead to the death of the cell. In this experiment, you will study the effect of changes in environmental conditions on the permeability of living beetroot cells.
Materials and Equipment:
Scalpel
Tweezers
Safety Goggles
Cork Borer
3-4 Beetroots
Small Beaker
Test tube Rack
7 Test Tubes (The Effect of Heat)
Hot Plate
10ml of 0.1M HCl
10ml of 0.01 M HCl
10ml of 0.001 M HCl
10ml of 0.001 M NaCl
10ml 0f 0.01 M NaCl
10ml of 0.1 M NaCl
10ml of tap water
9 Test Tubes (The Effect of pH)
Graduated Cylinder
6 Discs of Frozen Beetroots (3 per experiment)
10 ml of Acetone per experiment
Procedure:
The Effect of pH
1. Top and bottom of beetroot were cut off by scalpel. The cork borer was used to cut out about 3-4 cores, then each core was cut into uniform discs about 2mm thick.
2. Discs were rinsed in a small beaker of cool water for about 5 minutes.
3. Each stock solution of HCl, NaCl and tap water were measured in a graduated cylinder to 10ml, then poured into individually labeled test tubes.
4. Three beetroot discs were placed in each test tube with tweezers.
5. After 40 minute of waiting the colour and intensity was recorded.
The Effect of Freezing
1. 10 ml of room temperature water was measured with a graduated cylinder and poured into a labeled test tube.
2. Three frozen discs of beetroot were placed into the test tube of room temperature water.
3. After 40 minutes of waiting the colour and intensity