Beet Cell Lab
Beet Cell Lab
Purpose: The purpose of this Lab was to see how much pressure or stress the beet tissue would hold.
Hypothesis: I hypothesize that the higher % of the solvent that the higher the colour intensity will be shown on the ph colour scale. The lower the solvent then the less colour shown.
Materials:
*Test Tubes (4) *Cork Borer
*Beets
*Water
*1% Acetone
*50% Acetone
*1% Methanol
*50% Methanol
Procedure:
*Cut 4 uniform cylinders of beet using a cork borer with a 5-mm inside diameter. Trim each cylinder to 15-mm in length.
*Place these cylinders of beet tissues in a beaker and run tap water over then for 2 minutes to wash betacyanin from the injured cells on the surface.
*Discard the slightly coloured solution,
and place the 4 beets sections into 4 dry test tubes.
*Keep all beets at room temperature for 20 minutes and shake them occasionally. After 20 minutes, remove and discard the beet sections and observe the extent of membrane injury according to the amount of betacyanin that diffused into the organic solvent.
*Quantify the relative colour of each solution between 0 (colourless) and 10(darkest red).
Record your data in the table. Graph relative colour versus organic-solvent concentration according to a demonstration graph provided by your instructor.
Observations:
Tube Number Treatment Colour intensity
1 1% Acetone 2
2 50% Actetone 8
3 1% Methanol 2
4 50% Methanol 8 (1)
2a)The results my group got for part b which was the colour intensity of betacyaninun leaked from damaged membranes treated with various concentration of two organic solvents was that the stronger the substance the higher the colour intensity on the ph scale (0-10) with the 50% Methonal and Acetone ranking 8 in are opinion on the ph scale. Meanwhile the 1% Methonal and Acetone ranking 2 on the ph scale.
b) The solvents effects were basically the same on the beet tissues. 1% Methanol & Acetone had the same effect, conculiding they didn't really but that much pressure on the beet, which I believe, is why the colour wasn't as intense or dark. However the results with the 50% methanol & acetone was a lot darker, in are opinion giving it a 8 on the ph scale. This result is Probably because the methanol & acetone are a lot stronger then the 1%
c)well like in 2
3).
General conclusion:
1) How this situation may occur in some part of my body being exposed to conditions/substances that would cause membrane stress is the food we eat. Because in an every day situation we eat genetically modified food which are bodies can be exposed to some of these chemicals which can eventually cause stress to the membrane.
Purpose: The purpose of this Lab was to see how much pressure or stress the beet tissue would hold.
Hypothesis: I hypothesize that the higher % of the solvent that the higher the colour intensity will be shown on the ph colour scale. The lower the solvent then the less colour shown.
Materials:
*Test Tubes (4) *Cork Borer
*Beets
*Water
*1% Acetone
*50% Acetone
*1% Methanol
*50% Methanol
Procedure:
*Cut 4 uniform cylinders of beet using a cork borer with a 5-mm inside diameter. Trim each cylinder to 15-mm in length.
*Place these cylinders of beet tissues in a beaker and run tap water over then for 2 minutes to wash betacyanin from the injured cells on the surface.
*Discard the slightly coloured solution,
and place the 4 beets sections into 4 dry test tubes.
*Keep all beets at room temperature for 20 minutes and shake them occasionally. After 20 minutes, remove and discard the beet sections and observe the extent of membrane injury according to the amount of betacyanin that diffused into the organic solvent.
*Quantify the relative colour of each solution between 0 (colourless) and 10(darkest red).
Record your data in the table. Graph relative colour versus organic-solvent concentration according to a demonstration graph provided by your instructor.
Observations:
Tube Number Treatment Colour intensity
1 1% Acetone 2
2 50% Actetone 8
3 1% Methanol 2
4 50% Methanol 8 (1)
2a)The results my group got for part b which was the colour intensity of betacyaninun leaked from damaged membranes treated with various concentration of two organic solvents was that the stronger the substance the higher the colour intensity on the ph scale (0-10) with the 50% Methonal and Acetone ranking 8 in are opinion on the ph scale. Meanwhile the 1% Methonal and Acetone ranking 2 on the ph scale.
b) The solvents effects were basically the same on the beet tissues. 1% Methanol & Acetone had the same effect, conculiding they didn't really but that much pressure on the beet, which I believe, is why the colour wasn't as intense or dark. However the results with the 50% methanol & acetone was a lot darker, in are opinion giving it a 8 on the ph scale. This result is Probably because the methanol & acetone are a lot stronger then the 1%
c)well like in 2
3).
General conclusion:
1) How this situation may occur in some part of my body being exposed to conditions/substances that would cause membrane stress is the food we eat. Because in an every day situation we eat genetically modified food which are bodies can be exposed to some of these chemicals which can eventually cause stress to the membrane.