Benedict's Solution
PAMANTASAN NG LUNGSOD NG MAYNILA
(University of the City of Manila)
Intramuros, Manila
COLLEGE OF NURSING
Benedict’s Solution
Submitted by:
Marco Antonio A. Baltazar
BSN IV-3
Submitted to:
Prof. Marilyn Agravante
Community Health Nursing
Benedict's Solution
What is Benedict’s Solution?
Benedict's solution, deep-blue alkaline solution used to test for the presence of the aldehyde functional group, - CHO. The substance to be tested is heated with Benedict's solution; formation of a brick-red precipitate indicates presence of the aldehyde group. Since simple sugars (e.g., glucose) give a positive test, the solution is used to test for the presence of glucose in urine, a symptom of diabetes. One liter of Benedict's solution contains 173 grams sodium citrate, 100 grams sodium carbonate, and 17.3 grams cupric sulfate pentahydrate. It reacts chemically like Fehling's solution; the cupric ion (complexed with citrate ions) is reduced to cuprous ion by the aldehyde group (which is oxidized), and precipitates as cuprous oxide, Cu2O.
Benedict’s Test
Using Benedict’s test will reveal the presence of reducing sugars * It results in an orange-red precipitate. * The more reducing sugar there is present, the more precipitate will be formed, and the more Benedict’s solution (copper sulphate) will be used up. * The precipitate is filtered out then the concentration of the remaining solution can be measured. * This will tell you how much Benedict’s solution has been used up allowing you to estimate the concentration of reducing sugar in the original sample.
How Does A Benedict's Test Work?
Benedict's Solution is a method to determine glucose levels in people suffering from diabetes. It has copper sulphate in it, which reacts with sugars forming copper oxide. Copper oxide is reddish brown in color, therefore the redder the solution appears, the more copper oxide is present, which indicates higher levels of sugar in the solution.
(University of the City of Manila)
Intramuros, Manila
COLLEGE OF NURSING
Benedict’s Solution
Submitted by:
Marco Antonio A. Baltazar
BSN IV-3
Submitted to:
Prof. Marilyn Agravante
Community Health Nursing
Benedict's Solution
What is Benedict’s Solution?
Benedict's solution, deep-blue alkaline solution used to test for the presence of the aldehyde functional group, - CHO. The substance to be tested is heated with Benedict's solution; formation of a brick-red precipitate indicates presence of the aldehyde group. Since simple sugars (e.g., glucose) give a positive test, the solution is used to test for the presence of glucose in urine, a symptom of diabetes. One liter of Benedict's solution contains 173 grams sodium citrate, 100 grams sodium carbonate, and 17.3 grams cupric sulfate pentahydrate. It reacts chemically like Fehling's solution; the cupric ion (complexed with citrate ions) is reduced to cuprous ion by the aldehyde group (which is oxidized), and precipitates as cuprous oxide, Cu2O.
Benedict’s Test
Using Benedict’s test will reveal the presence of reducing sugars * It results in an orange-red precipitate. * The more reducing sugar there is present, the more precipitate will be formed, and the more Benedict’s solution (copper sulphate) will be used up. * The precipitate is filtered out then the concentration of the remaining solution can be measured. * This will tell you how much Benedict’s solution has been used up allowing you to estimate the concentration of reducing sugar in the original sample.
How Does A Benedict's Test Work?
Benedict's Solution is a method to determine glucose levels in people suffering from diabetes. It has copper sulphate in it, which reacts with sugars forming copper oxide. Copper oxide is reddish brown in color, therefore the redder the solution appears, the more copper oxide is present, which indicates higher levels of sugar in the solution.