1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion).
Which solute(s) were able to pass through the 20 MWCO membrane? None
According to your results, which solute had the highest molecular weight? Albumin
Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? NACI_
Using the data from Chart 1, explain the relationship between the rate of diffusion and the size of the solute. The smaller the solute particle, the greater the rate of diffusion.
Facilitated Diffusion
2. The following refer to Activity 2: Simulating Facilitated Diffusion.
Did any of the substances travel against their concentration gradient? Explain why or why not. No – In facilitated diffusion, substances can only move down their concentration gradient.
Using your results from Chart 2, what was the fastest rate of facilitated diffusion recorded? 0.0038 . Describe the conditions that were used to achieve this rate. 8mm glucose with 900 glucose carrier proteins.
Name two ways to increase the rate of glucose transport. Increase amount of glucose solute and increase number of glucose carrier membrane.
Did NaCl affect glucose transport? No
Did NaCl require a transport protein for diffusion? Why or why not? No because if its small size it can diffuse without a transport protein.
Osmotic Pressure
3. The following refer to Activity 3: Simulating Osmotic Pressure.
For NaCl, which MWCO membrane(s) provided for the net movement of water without movement of NaCl? 20
Explain how you determined this. (Hint: Correlate your results to the data in Chart 3.) Osmotic pressure building up because of non-diffusable NACI in the solute causes water to diffuse its concentration gradient.
For glucose, which MWCO membrane(s) provided for the net movement of glucose without net movement of water? 200
Explain how you determined this. No buildup of osmotic pressure shows glucose is able to diffuse down its concentration gradient.
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