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Biology Lab Write up

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Biology Lab Write up
Acids & Bases Lab Write Up
Lab 2

Introduction
There are many acids and bases all around us. An acid is known as a hydrogen ion donor (H¯), and a base is known as a hydrogen ion acceptor (H⁺). Acids and bases are represented by the pH scale. The pH scale shows the strength that an acid or base has. This scale ranges from 0-14, with 0-6 being acids, 8-14 being bases, and 7 being neutral, such as water. This is defined as the negative log of an ion atom (pH= -log10 [H⁺]) (Fultz, 2013). When a substance has an acid or base added it to it, it causes it to become more acidic or more of a base. If the acids or bases don’t cause much change in the pH, it is known as a buffer. A buffer is a compound that minimizes the pH fluctuation in the fluids of a living organism (Brooker et. al, 2013). In this organized experiment, we will be measuring the effects an acid and base has an effect on nine unknown solutions. Drops of an acid or base will be added to a solution to see the pH changes of the unknown solutions. These unknown solutions will be labeled A-J. Out of all these unknown materials we believe that by adding our base Sodium Hydroxide (0.5m NaOH) to the unknown substance F, it will not react and be labeled a buffer. We chose this as our buffer because it is clearer than the other unknown substances.
Methods
In this experiment we used a Vernier computer interface to calculate the pH change of each substance we were given to see which one it effected the most and least. In a 250-mL beaker we filled it up with about 100-mL of distilled water to keep the pH electrode from drying out. Before starting out the lab, we had to obtain gloves and eye ware to keep the acids and bases from contacting sensitive parts of the body. We then labeled our 50-mL beaker “basic”, because we were assigned a base. After labeling it basic, we then placed 20-mL of distilled water in the beaker. The pH should stay the same of the distilled

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