Laboratory 2 Report: Enzyme Catakysis 1. Title • The effect that temperature, pH, time, enzyme concentration, and substrate concentration has on Enzyme Catalysis. 2. Abstract • Assess the general functions and activities of enzymes. Analyze the relationship between the structure and function of enzymes. Understand the concept of initial reaction rates of enzymes. Change the temperature, pH, enzyme concentration, substrate concentration, and time and record the effects of the enzyme catalysis. Explain how environmental factors affect the rate of enzyme-catalyzed reaction. Explain why catalysis activity decreased over time. 3. Introduction • An enzyme is a specific organic catalyst (substance that speeds up reaction without being used up in the experiment) in which it binds on to a specific substrate (reactant) at the active site and lowers the activation energy required to perform that reaction. Enzymes are important biologically because, for example, the human body performs certain reactions that keep us alive and most of them react at 37 degrees Celsius (body temperature. In each of these reactions, there is an enzyme that catalyzes these reactions to lower the amount of energy needed to perform this reaction. If enzymes didn’t exist then body temperature wouldn’t be enough to perform any of these reactions properly. Enzymes have certain conditions in which it will work in. For instance, most enzymes react at certain temperatures (varies in each enzyme), most enzymes react from a pH of 6- 8, and react faster or slower according to the amount of substrate present. Altering temperature, pH level, and concentrations of enzyme and substrates may cause absolute no change to the activation energy in which the enzyme would have been denatured. In addition to these environmental factors, other molecules may react with an enzyme. These may have great to no effect on the enzymes. Those
Laboratory 2 Report: Enzyme Catakysis 1. Title • The effect that temperature, pH, time, enzyme concentration, and substrate concentration has on Enzyme Catalysis. 2. Abstract • Assess the general functions and activities of enzymes. Analyze the relationship between the structure and function of enzymes. Understand the concept of initial reaction rates of enzymes. Change the temperature, pH, enzyme concentration, substrate concentration, and time and record the effects of the enzyme catalysis. Explain how environmental factors affect the rate of enzyme-catalyzed reaction. Explain why catalysis activity decreased over time. 3. Introduction • An enzyme is a specific organic catalyst (substance that speeds up reaction without being used up in the experiment) in which it binds on to a specific substrate (reactant) at the active site and lowers the activation energy required to perform that reaction. Enzymes are important biologically because, for example, the human body performs certain reactions that keep us alive and most of them react at 37 degrees Celsius (body temperature. In each of these reactions, there is an enzyme that catalyzes these reactions to lower the amount of energy needed to perform this reaction. If enzymes didn’t exist then body temperature wouldn’t be enough to perform any of these reactions properly. Enzymes have certain conditions in which it will work in. For instance, most enzymes react at certain temperatures (varies in each enzyme), most enzymes react from a pH of 6- 8, and react faster or slower according to the amount of substrate present. Altering temperature, pH level, and concentrations of enzyme and substrates may cause absolute no change to the activation energy in which the enzyme would have been denatured. In addition to these environmental factors, other molecules may react with an enzyme. These may have great to no effect on the enzymes. Those