Carbohydrates, Proteins, Lipids, and Nucleic Acid
Carbohydrates, Proteins, lipids, and nucleic Acid
Lab Exercise 6
Date: 9/17/12
Bio 102-11
Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.
Background
Most organic compounds in living organisms are carbohydrates, proteins, lipids, and nucleic acids they are called macromolecules. Each of these macromolecules is made of smaller subunits. These subunits are linked by dehydration synthesis. Dehydration synthesis is an energy requiring process in which a molecule of water is removing and the two subunits are boded covalently. This energy releasing process is called hydrolysis. The subunits of macromolecules are held together by covalent bonds and have different structure and properties. For example, lipids made of fatty acids have many C-H bonds and relatively little oxygen; while proteins are made of amino acids have groups NH2 and carboxyl groups.
In an experiment we have two or more treatments, an unknown solution to be identified, and two controls to provide standard for comparison, a positive controls and a negative control. The positive control contain the variable for which you are testing, it react positively and demonstrates that test’s ability to detect what you expect. For example if you testing for protein is a solution known to contain protein, a positive reaction shows that your test reacts correctly, it ‘also shows you what a positive test look like. A negative control does not contain the variable for which you are searching. It contains only the solvent often distilled water with no solute and does not react in the test. A negative control shows you what a negative result looks like.
Carbohydrates are molecules of carbon, hydrogen and oxygen in a ratio of 1:2:1 the chemical formula for glucose is C6 H12 O6.
Carbohydrates are
Lab Exercise 6
Date: 9/17/12
Bio 102-11
Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.
Background
Most organic compounds in living organisms are carbohydrates, proteins, lipids, and nucleic acids they are called macromolecules. Each of these macromolecules is made of smaller subunits. These subunits are linked by dehydration synthesis. Dehydration synthesis is an energy requiring process in which a molecule of water is removing and the two subunits are boded covalently. This energy releasing process is called hydrolysis. The subunits of macromolecules are held together by covalent bonds and have different structure and properties. For example, lipids made of fatty acids have many C-H bonds and relatively little oxygen; while proteins are made of amino acids have groups NH2 and carboxyl groups.
In an experiment we have two or more treatments, an unknown solution to be identified, and two controls to provide standard for comparison, a positive controls and a negative control. The positive control contain the variable for which you are testing, it react positively and demonstrates that test’s ability to detect what you expect. For example if you testing for protein is a solution known to contain protein, a positive reaction shows that your test reacts correctly, it ‘also shows you what a positive test look like. A negative control does not contain the variable for which you are searching. It contains only the solvent often distilled water with no solute and does not react in the test. A negative control shows you what a negative result looks like.
Carbohydrates are molecules of carbon, hydrogen and oxygen in a ratio of 1:2:1 the chemical formula for glucose is C6 H12 O6.
Carbohydrates are