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Distilled Water, Carbohydrate For Macromolecules?

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Distilled Water, Carbohydrate For Macromolecules?
In this lab activity students performed an experiment with the use of distilled water and baking soda solutions as control. The experimental controls are accuracy indicators of laboratory results. Thus, controls are important in a lab because the observation results are dependent on the controls. The experimental results can be determined to be valid when it is compared with the control solution. The higher the similarity between the results and the control solution, the more accurate the investigation is. Therefore, since the controls used in the lab are not one of the macromolecules; such as, carbohydrates, lipids or proteins, makes the controls ideal for this lab. In this lab, the controls are important because their role is to demonstrate …show more content…
The solvents used in this test were: in water, baking soda, vegetable oil, skim milk, whip cream, and the unknown solution. There were two ways for the students to determine which molecules were present for each substances tested. First, the presence of lipids turns the indicator from a pink to red colour (Testing for Macromolecules, 2015). Secondly, the control mixture provided great examples of negative result. The negative result occurs when the colour mixture become dark red (Identifying Macromolecules). The presence of the negative result will also give an identifiable positive result. The positive result ranged colours from reddish orange (Identifying Macromolecules). Based on class observations (refer to table two), the only solvents that contained lipids were vegetable oil and slightly skim milk. Subsequently, the Sudan IV solution has the ability to liquefy in lipids, except with water. Thus, if it reacts with lipids a colour change will occur. It will turn into red colour which is a positive result in this case. As discussed in class, unsaturated fat is a lipid that exists as a liquid when it is at room temperature (Your Dictionary). Plant oils are contained with unsaturated fats and vegetable oil is an example of polyunsaturated fats (Omega 3 fatty acids). Hence, it makes sense that vegetable oil reacted with Sudan since it is classified as a type of lipid. As for the rest of the result, most of the substances turned light pink. The unknown solution undergo a colour change to a light pink colour. This could indicate that there were lipids contained in the substance. All in all, the remaining results were expected since the substances changed colours to almost red colour when Sudan IV is added in vegetable oils, skim milk, and whip cream; these all contain lipids. The second part of the lipid test was the paper towel experiment. In

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