Cell Membrane
1 The Effect of Molecular Weight on the Rate of Diffusion of Substances1
Alexander Ken Libranza Group 1 Sec. A – 1L
March 6, 2012
A scientific paper submitted in partial fulfillment of the requirements in General Biology I laboratory under Prof. Cheryl M. Talde, 2nd sem., 2011-2012.
1
2 ABSTRACT
The effect of molecular weight on the rate of diffusion was assessed using two tests: the glass tube test and the agar-water gel test. In the glass tube set-up, two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH 4OH, M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm), resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl, M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed of a petri dish of agar-water gel containing three wells. Drops of potassium permanganate (KMnO4), potassium dichromate (K2Cr2O7) and methylene blue were simultaneously introduced to each well. Methylene blue, having the largest molecular weight, displayed the smallest diameter (18 mm) and diffused at the slowest rate (0.3668 mm/min.). Thus, the higher the molecular weight, the slower the rate of diffusion.
INTRODUCTION
A substance in the gaseous or liquid state consists of molecules or atoms that are independent, rapid, and random in motion. These molecules frequently collide with each other and with the sides of the container. In a period of time, this movement results in a uniform distribution of the molecules throughout the system. This process is called diffusion (Everett and Everett, 2007). Diffusion occurs naturally, with the net movement of particles flowing from an area of high concentration to an area of low concentration. Net diffusion can be restated as the movement of particles along the concentration gradient.
3 According to Meyertholen (2007), there
Alexander Ken Libranza Group 1 Sec. A – 1L
March 6, 2012
A scientific paper submitted in partial fulfillment of the requirements in General Biology I laboratory under Prof. Cheryl M. Talde, 2nd sem., 2011-2012.
1
2 ABSTRACT
The effect of molecular weight on the rate of diffusion was assessed using two tests: the glass tube test and the agar-water gel test. In the glass tube set-up, two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH 4OH, M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm), resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl, M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed of a petri dish of agar-water gel containing three wells. Drops of potassium permanganate (KMnO4), potassium dichromate (K2Cr2O7) and methylene blue were simultaneously introduced to each well. Methylene blue, having the largest molecular weight, displayed the smallest diameter (18 mm) and diffused at the slowest rate (0.3668 mm/min.). Thus, the higher the molecular weight, the slower the rate of diffusion.
INTRODUCTION
A substance in the gaseous or liquid state consists of molecules or atoms that are independent, rapid, and random in motion. These molecules frequently collide with each other and with the sides of the container. In a period of time, this movement results in a uniform distribution of the molecules throughout the system. This process is called diffusion (Everett and Everett, 2007). Diffusion occurs naturally, with the net movement of particles flowing from an area of high concentration to an area of low concentration. Net diffusion can be restated as the movement of particles along the concentration gradient.
3 According to Meyertholen (2007), there
Cited: Chang, R. 1998. Chemistry. 6th ed. Boston: James M. Smith. Everett, G.W and G.W. Everett, Jr. (n.d.). Diffusion of Gases and Graham’s Law. Retrieved Aug. 14, 2011 from http://www.cerlabs.com/experiments/ 1087540412X.pdf Meyertholen, E. (n.d.) Diffusion. Retrieved Aug. 14, 2011 from http://www.austincc.edu/ ~emeyerth/diffuse2.htm Nave, R. 2008. Diffusion. Retrieved Aug. 14, 2011 from http://hyperphysics.phyastr.gsu.edu/hbase/kinetic/diffus.html Weiss, G. 1994. Aspects and Applications of the Random Walk. Amsterdam, Netherlands: North-Holla Duka, IMA and Diaz, MGQ (n.d.) Biology I Laboratory Manual: An Investigative Approach 9th Edition. UP Los Baῆos, College, Laguna, Philippines. 2009