Spring 2015 Study Guide 1
STUDY GUIDE FOR PRACTICAL #1 Spring 2015
Bio 115 (Biology I Lab)
1. Be able to identify the parts of the compound light microscope. Know the functions of those parts.
2. Understand how to focus a slide/specimen under the microscope. Be familiar with the steps taken to focus under low and high power. Be ready to define and calculate total magnification and know what happens to the field of view when the magnification is increased.
3. Understand how to measure the field diameter using a micrometer. Be able to predict (estimate) the length of objects in the field of view using that knowledge.
4. Be able to identify all the functional groups that you built in lab. Be ready to identify the models, name the functional group, and the molecular/scientific formula (ie. Methyl group, - CH3)
5. Be able to identify all the organic and inorganic molecules that you built in lab. Be ready to identify the models, name the molecule, and the molecular/scientific formula (ie. ethyl alcohol, CH3CH2OH). You should know the difference between organic and inorganic molecules.
6. Be familiar with the biochemical tests performed in lab-including the Millon test, Biuret test, Benedict’s test, Iodine test, Grease spot and Sudan Dye test. Know what each test is used for- protein (peptide bonds or tyrosine groups), glucose, sucrose, starch or lipids. You should know the chemicals/reagents in each test and the results that would indicate a positive or negative for each biochemical.
7. Define and understand the concepts behind common processes including diffusion, osmosis, active transport, Brownian movement and plasmolysis.
8. Understand the methods by which materials move through plasma membranes including diffusion and osmosis.
9. Be ready to define concentration gradient and turgor pressure.
10. Understand how differences in the molecular weight of a substance affect the rate of movement in diffusion.
11. Be ready to predict what will happen to a blood cell when
Bio 115 (Biology I Lab)
1. Be able to identify the parts of the compound light microscope. Know the functions of those parts.
2. Understand how to focus a slide/specimen under the microscope. Be familiar with the steps taken to focus under low and high power. Be ready to define and calculate total magnification and know what happens to the field of view when the magnification is increased.
3. Understand how to measure the field diameter using a micrometer. Be able to predict (estimate) the length of objects in the field of view using that knowledge.
4. Be able to identify all the functional groups that you built in lab. Be ready to identify the models, name the functional group, and the molecular/scientific formula (ie. Methyl group, - CH3)
5. Be able to identify all the organic and inorganic molecules that you built in lab. Be ready to identify the models, name the molecule, and the molecular/scientific formula (ie. ethyl alcohol, CH3CH2OH). You should know the difference between organic and inorganic molecules.
6. Be familiar with the biochemical tests performed in lab-including the Millon test, Biuret test, Benedict’s test, Iodine test, Grease spot and Sudan Dye test. Know what each test is used for- protein (peptide bonds or tyrosine groups), glucose, sucrose, starch or lipids. You should know the chemicals/reagents in each test and the results that would indicate a positive or negative for each biochemical.
7. Define and understand the concepts behind common processes including diffusion, osmosis, active transport, Brownian movement and plasmolysis.
8. Understand the methods by which materials move through plasma membranes including diffusion and osmosis.
9. Be ready to define concentration gradient and turgor pressure.
10. Understand how differences in the molecular weight of a substance affect the rate of movement in diffusion.
11. Be ready to predict what will happen to a blood cell when