Observing Bacteria and Blood- Lab #1
Title: Observing Bacteria and Blood- Lab #1
Purpose: Being able to learn how to correctly use a microscope and the oil immersion lens to be able to see the prepared slides. Also to learn how to prepare my own yogurt and blood slides.
Procedure: First, set up the microscope. Clean the ocular lenses and objectives with lens paper. Then pace the prepared e slide on the stage and make adjustments. Turn the rotating nosepiece until the 10x objective is above the ring of light coming through the slide. Move the slide using the X and Y stage knobs until the specimen is within the view. Adjust the focus by looking into the eyepiece and focusing the specimen with the coarse then fine focus knobs. Adjust diaphragm until there is sufficient light passing through the specimen. Do that with the next different prepared slides with 10x and 40x objectives. Place a drop of oil on the slide and rotate the oil immersion objective, 100x, into the oil, then past the oil and back. Use the fine focus to bring the object into focus.
Second, find a sealable container made of glass or plastic. Clean the container thoroughly with soap and then rinse the container several times to remove the soap. Place a teaspoon of yogurt in the container. Cover the container and place it in a dark, warm area. Leave the container for 12–24 hours. Take a sample of yogurt from the container and place the sample on a clean slide. Observe the bacteria under the microscope at 10x, 40x, and 100x oil immersion. Setting should be low. Compare your observations of the fresh, live slide to the prepared, stained slide. Third, poke the inside of your finger with the lancet. Squeeze your finger to place a drop of blood on a clean slide. Drop the blood toward one end of a slide. Tilt the cover slip toward the drop. Lay the cover slip flat across the blood smear.
Data Table:
Slide
10x
40x
100x
Bacteria bacillus form
Tiny dot
Dots looking more like a shape
Small rods
Bacteria coccus form
Purpose: Being able to learn how to correctly use a microscope and the oil immersion lens to be able to see the prepared slides. Also to learn how to prepare my own yogurt and blood slides.
Procedure: First, set up the microscope. Clean the ocular lenses and objectives with lens paper. Then pace the prepared e slide on the stage and make adjustments. Turn the rotating nosepiece until the 10x objective is above the ring of light coming through the slide. Move the slide using the X and Y stage knobs until the specimen is within the view. Adjust the focus by looking into the eyepiece and focusing the specimen with the coarse then fine focus knobs. Adjust diaphragm until there is sufficient light passing through the specimen. Do that with the next different prepared slides with 10x and 40x objectives. Place a drop of oil on the slide and rotate the oil immersion objective, 100x, into the oil, then past the oil and back. Use the fine focus to bring the object into focus.
Second, find a sealable container made of glass or plastic. Clean the container thoroughly with soap and then rinse the container several times to remove the soap. Place a teaspoon of yogurt in the container. Cover the container and place it in a dark, warm area. Leave the container for 12–24 hours. Take a sample of yogurt from the container and place the sample on a clean slide. Observe the bacteria under the microscope at 10x, 40x, and 100x oil immersion. Setting should be low. Compare your observations of the fresh, live slide to the prepared, stained slide. Third, poke the inside of your finger with the lancet. Squeeze your finger to place a drop of blood on a clean slide. Drop the blood toward one end of a slide. Tilt the cover slip toward the drop. Lay the cover slip flat across the blood smear.
Data Table:
Slide
10x
40x
100x
Bacteria bacillus form
Tiny dot
Dots looking more like a shape
Small rods
Bacteria coccus form