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Microbiology Unit 20 Lab Report

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Microbiology Unit 20 Lab Report
Microbiology 20 Laboratory Final Exam Guidelines
The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or
Thursday December 6th (section 3253) and consist of an open book portion given in CMS 105 followed by a practical portion (closed book) to be given in the lab (CMS 002). You will not need a Scantron.
For the open book test, be sure to bring any resources you may need including your lab manual, lab notebook, safety guidelines and handouts. For the practical portion there will be 28 stations set up in the laboratory with something to observe and one or more questions at each station.
You will have 1 minute per station after which you will need to move to the next station. You may also be asked to perform various laboratory skills (see below). In preparation, be sure to
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understand and be able to recognize and/or interpret the following:
Lab 3-1: Light Microscope
Basic microscope parts/functioning
Identify prepared slides of the following organisms:
Labs 3-3: Eukaryotic Organisms
Identify prepared slides of the following organisms and/or structures:
Protozoa: Amoeba, Paramecium, Plasmodium, Trypanosoma
Algae: Diatoms, Spirogyra, Volvox
Helminths: Tapeworm, fluke
Fungi: Methylene blue stain of yeast cells (Saccharomyces), sporangiospores of Rhizopus, conidiospores of Penicillium
Staining Labs (3-6, 3-7, 3-8, and 3-9) – Identify and correctly interpret the following:
Lab 3-6: Gram stain
Lab 3-7: Acid Fast stain
Lab 3-8: Capsule stain
Lab 3-9: Endospore stain
Metabolic Tests:
Lab 5-1: O-F Glucose (aerobic versus fermentative organisms)
Lab 5-2: Phenol Red broth for sugar fermentation (acid and/or gas)
Lab 5-3: MR-VP: Methyl red and Voges Proskauer tests
Lab 5-4: Catalase
Lab 5-5: Oxidase
Lab 5-6: Nitrate Reduction (to nitrite, nitrous oxide or nitrogen gas)
Lab 5-7: Citrate

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