subspinosus. Check that occurs correlation between genetic and geographic distance. Estimate the relationship between pairs of individuals sampled in order to support the development of efficient strategies for in situ conservation of the species. To elucidate these proposals work has been structured into five chapters: chapter 01 describes the detection, isolation and genetic characterization of Toxoplasma gondii isolates of C. subspinosus; chapter 02 is identify the tick species Amblyomma longirostre that parasites sampled C. subspinosus of individuals indicating the presence of Rickettsia bellii and Rickettsia amblyommii; chapter 03 was carried out to identify pathogenic and nonpathogenic bacteria present in the modified spines of C. subspinosus; chapter 04 deals with the development and characterization through next-generation sequencing (NGS), 30 unpublished microsatellite markers, polymorphic and specific to the species; and chapter 05, developed markers were used as molecular tools to assess level of structure and genetic diversity, gene flow, allelic composition of C. subspinosus. With biological knowledge obtained at end study can indicate measures for control of possible diseases caused by zoonotic agents detected and subsidize conservation
subspinosus. Check that occurs correlation between genetic and geographic distance. Estimate the relationship between pairs of individuals sampled in order to support the development of efficient strategies for in situ conservation of the species. To elucidate these proposals work has been structured into five chapters: chapter 01 describes the detection, isolation and genetic characterization of Toxoplasma gondii isolates of C. subspinosus; chapter 02 is identify the tick species Amblyomma longirostre that parasites sampled C. subspinosus of individuals indicating the presence of Rickettsia bellii and Rickettsia amblyommii; chapter 03 was carried out to identify pathogenic and nonpathogenic bacteria present in the modified spines of C. subspinosus; chapter 04 deals with the development and characterization through next-generation sequencing (NGS), 30 unpublished microsatellite markers, polymorphic and specific to the species; and chapter 05, developed markers were used as molecular tools to assess level of structure and genetic diversity, gene flow, allelic composition of C. subspinosus. With biological knowledge obtained at end study can indicate measures for control of possible diseases caused by zoonotic agents detected and subsidize conservation