Characterization Of The S2 Vnp Loaded Liquid Nnds Case Study
3.3. Characterization of the S2 VNP loaded liquid SNEDDS
3.3.1. Assessment of self-emulsification efficiency
The emulsification time for S2, which is the time needed for SNEDDS to form homogeneous transparent mixture upon dilution, was 12 sec when 1 gm of VNP SNEDDS was added to 200 ml of 0.1 N HCl. When the same experiment was performed but by using 500 ml distilled water, the time required for the complete disappearance of SNEDDS was 14 sec. The emulsification in both media is under grade A of the grading system that is previously mentioned which means that the emulsion was formed rapidly (within 1 min) and it had a clear appearance. Khoo et al. (Khoo et al., 1998) assigned a max time of 2 min as an efficiency parameter for the self-emulsification process and this was proposed in order to ensure the rapid release of drug after aqueous dilution in the stomach under human peristaltic movement. …show more content…
Robustness to dilution
It was found that the dilution had no effect on the clarity of the diluted liquid system. All the dilutions in the used media at all used ratios were clear with neither phase separation nor drug precipitation. This ensured that the problem of VNP precipitation in small intestine was solved and the presence of the drug within the SNEDDS kept it in a solubilized form whatever the media is. 3.3.3. Dye solubilization test
There was a spontaneous dispersion of the water soluble blue dye upon sprinkling onto the surface of prepared nano-emulsion. The rapid incorporation of the water soluble dye into the system indicated that the continuous phase was water, which signified the formation of O/W nano-emulsion.
3.3.4. Transmittance
The %T of the prepared diluted emulsion was determined spectrophotometrically at 630 nm to check the clarity of the prepared liquid SNEDDS. Mean %T of the diluted formulation was 98.83 % ± 0.93. This means a clear SNEDDS with small drug particle size. 3.3.5. Droplet size analysis and zeta potential
3.3.1. Assessment of self-emulsification efficiency
The emulsification time for S2, which is the time needed for SNEDDS to form homogeneous transparent mixture upon dilution, was 12 sec when 1 gm of VNP SNEDDS was added to 200 ml of 0.1 N HCl. When the same experiment was performed but by using 500 ml distilled water, the time required for the complete disappearance of SNEDDS was 14 sec. The emulsification in both media is under grade A of the grading system that is previously mentioned which means that the emulsion was formed rapidly (within 1 min) and it had a clear appearance. Khoo et al. (Khoo et al., 1998) assigned a max time of 2 min as an efficiency parameter for the self-emulsification process and this was proposed in order to ensure the rapid release of drug after aqueous dilution in the stomach under human peristaltic movement. …show more content…
Robustness to dilution
It was found that the dilution had no effect on the clarity of the diluted liquid system. All the dilutions in the used media at all used ratios were clear with neither phase separation nor drug precipitation. This ensured that the problem of VNP precipitation in small intestine was solved and the presence of the drug within the SNEDDS kept it in a solubilized form whatever the media is. 3.3.3. Dye solubilization test
There was a spontaneous dispersion of the water soluble blue dye upon sprinkling onto the surface of prepared nano-emulsion. The rapid incorporation of the water soluble dye into the system indicated that the continuous phase was water, which signified the formation of O/W nano-emulsion.
3.3.4. Transmittance
The %T of the prepared diluted emulsion was determined spectrophotometrically at 630 nm to check the clarity of the prepared liquid SNEDDS. Mean %T of the diluted formulation was 98.83 % ± 0.93. This means a clear SNEDDS with small drug particle size. 3.3.5. Droplet size analysis and zeta potential