chemistry
OBJECTIVES:
To determine the maximum wavelength of potassium permanganate.
To plot the calibration curve of potassium permanganate.
To determine the concentration of an unknown solution of potassium permanganate.
INTRODUCTION:
UV Spectrophotometer has 4 main components which is the UV light source, the sample, detector and the processor/recorder. Spectrophotometry is a technique that uses the absorbance of light by an analyte (the substance to be analyzed) at a certain wavelength to determine the analyte concentration. Useful wavelengths for spectrophotometry range from 185 to 3,000 nm. Spectroscopy is one of the most powerful analytical techniques in modern science. Spectroscopy works by correlating the concentration of a species in solution to the amount of light it absorbs. It included the observations of absorption or emission of electromagnetic radiation resulting from transitions of atoms or molecules from one energy level to other level. The spectrophotometer measures how much light is absorbed at a given wavelength. When an atom or molecule absorbs energy, electrons are promoted from their ground state to an excited state. When a molecule at a ground state absorbs energy, it called transition to a higher energy state.The higher energy state can be said as excited state. In a molecule, the atoms can rotate and vibrate with respect to each other. These vibrations and rotations also have discrete energy levels, which can be considered as being packed on top of each electronic level.
UV/Vis spectroscopy is routinely used in analytical chemistry for thequantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied. Solutions of transition metal ions can be colored (absorb visible light) because the electrons within the metal atoms can be excited from one electronic state
To determine the maximum wavelength of potassium permanganate.
To plot the calibration curve of potassium permanganate.
To determine the concentration of an unknown solution of potassium permanganate.
INTRODUCTION:
UV Spectrophotometer has 4 main components which is the UV light source, the sample, detector and the processor/recorder. Spectrophotometry is a technique that uses the absorbance of light by an analyte (the substance to be analyzed) at a certain wavelength to determine the analyte concentration. Useful wavelengths for spectrophotometry range from 185 to 3,000 nm. Spectroscopy is one of the most powerful analytical techniques in modern science. Spectroscopy works by correlating the concentration of a species in solution to the amount of light it absorbs. It included the observations of absorption or emission of electromagnetic radiation resulting from transitions of atoms or molecules from one energy level to other level. The spectrophotometer measures how much light is absorbed at a given wavelength. When an atom or molecule absorbs energy, electrons are promoted from their ground state to an excited state. When a molecule at a ground state absorbs energy, it called transition to a higher energy state.The higher energy state can be said as excited state. In a molecule, the atoms can rotate and vibrate with respect to each other. These vibrations and rotations also have discrete energy levels, which can be considered as being packed on top of each electronic level.
UV/Vis spectroscopy is routinely used in analytical chemistry for thequantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied. Solutions of transition metal ions can be colored (absorb visible light) because the electrons within the metal atoms can be excited from one electronic state
References: S: Harris, Daniel C. Sixth Edition Quantitative Chemical Analysis. Pg. 408-409. New York: W.H. Freeman and Company, 2003. Raymond C. Tenth Edition CHEMISTRY. Pg. 147-149. New York: McGraw-Hill and Company, 2010.