Gregorio, Justin Edrik A.
March 2013
Department of Chemical Engineering, Faculty of Engineering
University of Santo Tomas
España, Manila
Abstract The purpose of this analytical laboratory experiment is to determine the unknown concentration of potassium permanganate (KMnO4) solution by finding its absorbance through the use of spectrophotometer. The preparation of four known concentration of KMnO4 was done namely, 2.00×10-4M, 1.50×10-4M, 1.00×10-4M, 5.00×10-5M, respectively and is to be place on the spectrophotometer with the unknown and distilled water for the determination of each concentration’s absorbance. As the concentration is proportional with the absorbance of the solution, to determine the concentration of the solution is possible by drawing a graph of concentration against the absorbance. At the end of the experiment, the group found the concentration of the unknown sample is 2.50×10-4M.
I. Introduction
Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength. This measurement can also be used to measure the amount of a known chemical substance. Spectrophotometry is one of the most useful methods of quantitative analysis in various fields such as chemistry, physics, biochemistry, material and chemical engineering and clinical applications. A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution. With the spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected.
In this experiment, we use the spectrophotometer to determine the unknown concentration of potassium permanganate
References: * Christy P. 2010. The History of Spectrophotometry. http://www.ehow.com/about_6595173_history-spectrophotometry.html. * David B * Dr. Laminar. 1998. Spectrophotometric Analysis. http://www.public.asu.edu/~lwmays/classes/cee341/lab_example.pdf. * M.C