Chlorella Vulgaris Lab Report

Chlorella vulgaris was first obtained and placed onto a wet mount at 400X using a light microscope to take measurements on size of the organism. With a stage micrometer, a cell size of 5 micrometers was recorded (5µm). It was observed that the cells were globularly shaped and green in color with some movement while floating in the surrounding fluid. Chlorella vulgaris was placed in media for optimum growth potential. DNA isolation was used to isolate chloroplast, mitochondrial and nuclear DNA. The source of DNA for this procedure was a sample of this organism from a liquid broth culture previously prepared. The DNA sample was then added to a primer loading dye mixture in preparation for PCR. The primers used in this procedure serve as a starting