Standard solutions Stock solutions of LVD, CBD and ETC containing to 1.0 mg mL-1 were prepared separately by dissolving 50.0 mg in 50 mL of methanol by sonication. The stock solutions were diluted with the mobile phase to prepare working solutions. For each drug, the solutions were stable for 7 days when preserved in the refrigerator …show more content…
The Ethics of Human Committee of the Faculty of Pharmacy, University of Mansoura, confirmed the survey (2016-65 on 29/10/2016 code number). The agreements of the volunteers were obtained prior to the experiment. An investigation of LVD and CBD or LVD, CBD and ETC was achieved on two groups of three healthy males (normal kidney, liver functions and cardiogram), acquainted adult volunteers (about 35 years), with no previous history of sensitivity for LVD, CBD or ETC. Every volunteer was informed for refraining from whole medicaments for fourteen days prior to administration of the drugs and moreover through investigation. Furthermore, all volunteers were informed for administration of only one tablet of Sinemet® or Stalevo® for either group. Blood samples were withdrawn from volunteers at different time intervals at 0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 5.0, 6.0, 7.0 and 8.0 hours into tubes containing 0.5 ml of 2% EDTA solution to inhibit blood coagulation. Plasma was obtained from blood through centrifugation for 20 min at 3500 resolution per minute. Plasma supernatant was transferred to two groups of 5.0 mL volumetric flasks. The procedure was performed as described under “LVD, CBD and ETC analysis spiked human plasma” and the peak area of LVD, CBD and ETC was measured. By using the corresponding equations of regression, the accurate concentration of drugs in-vivo was