Chromatography Rf Values And Aerobic Metabolism

BIOL 1F90
Experiment #2
Aerobic and Anaerobic Metabolism

Caleb Coolen
5407754
Lab Section 21

Experiment Performed: October 10, 2013
Lab Partners: John Elgersma, Sandra-Lynn Plourde & Taylor Spratt

Abstract:
Materials and Methods:
Methods and materials used in this experiment can be found in the BIOL 1F90 Laboratory Manual (Martin, 2013), Experiment # 1, pages 2-5.

Results:

Table 1: Culture Sample Chromatography Rf Values

Sample of Spot
Distance from Origin (mm)
Solvent Front (mm)
Rf Value
Color
A
6.0
57.0
0.22
Bluish green
B
17.5
56.0
0.105
Dark green blue
C
4.0
57.0
0.070
Greenish blue
D
4.0
47.0
0.085
Green
E
10.5
56.5
0.186
Light blue

Figure 1: Chromatography- TLC sheet
From left to right- acetic acid, citric acid,
Figure 3: Incorrect Ethanol Well Assay Results
This solution is still green because our group did not do it correctly. Thus different measures were used.

Table 3: Ethanol Well Plate Assay Results from Figure 4

Sample
Concentration (M)
A
2.4
B
2.4
C
0.0
D
1.2
E
2.4

Figure 4: Correct Ethanol Well Plate Assay Results
The column on the left for this figure 3 and the first well at the bottom of the second column are the sample solutions containing different ethanol concentrations so the remainder wells can be compared to them. Alexa Plato did this assay

Discussion: Different samples used different metabolic pathways. In the samples that had higher concentrations of pyruvic acid, glycolysis was present. The yeast organism could only oxidize the sugar so far because oxygen was not present and thus pyruvate did not enter the citric acid cycle but instead turned to acid. The process of alcohol fermentation does this as ethanol and carbon dioxide are the eventual bi-products. Where citric acid and acetic acid were seen, the organism could go a bit farther due to the presence of oxygen amounts and enter into the citric acid