Introduction
In this lab we will establish a difference between the two types of cell transport mechanisms and their permeability. The first type of cell transport is passive processes which are driven by concentration or pressure differences in the interior and exterior of the cell. The second type is active processes which use energy known as ATP to power the transport. There are two main types of passive processes called diffusion and filtration. This can be split into different types such as simple diffisuion, facilitated diffusion, osmosis and filtration.
The objective of these experiments is to provide information on the passage of solutes and water through semi permeable membranes and relate them back to the study of actual living membranes in the human body. The purpose is to better understand how and why membranes use certain types of passive or active processes to move solutes and water across its membrane.
Simple diffusion is the net movement of substances from a region of high concentration to a region of low concentration. It will move down its concentration gradient meaning the molecules will eventually become evenly distributed throughout the environment. In this experiment NaCl, urea, albumin and glucose will be used to show how different substances move differently across the membrane. If a substance is not able to go across a membrane then the size of particles are to large to pass through, but other particles depending on size should still be able to pass through.
Facilitated diffusion is also the net movement of substances moving down their concentration gradient, but with the help of carrier proteins. Which are proteins that bind to substance or molecule and help it transport across the membrane. In this experiment we will use different concentrations of glucose to show the affects carrier proteins have on its