Purpose
The purpose of this experiment is to have a better understanding of the subject matter and to understand the difference between active and passive cellular transport. It will also help to define diffusion and describe the process that occurs when certain solutes come in contact and are either stopped or move through the plasma membrane.
Hypothesis
I believe the plasma membrane is selective about what is able to and not able to pass through it. It stops undesirable substances from passing and it will take in nutrients that are acceptable or needed. Materials & Methods Materials: Online Pearson 's Lab.
Methods:
Follow the steps and procedures laid out for me. Results
Experiment Results
Predict Question:
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer : a. Yes, but very slowly.
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mole.
Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer : c. glucose only
Stop & Think Questions:
The reason sodium chloride didn 't diffuse left to right is that
You correctly answered: c. the membrane pore size was too small.
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
You correctly answered: d. glucose and albumin are both too large to pass.
The rate of diffusion for urea
You correctly answered: b. is slower than that for sodium because urea is a larger molecule.
Experiment Data:
Solute MWCO Solute Concentration Average Diffusion Rate
Na+ Cl- 20 9.00 0.0000
Urea 20 9.00 0.0000
Na+ Cl- 50 9.00 0.0150
Na+ Cl- 50 18.00