Microbial Metabolism
Chapter 8 and 9.3
I.
Enzymes: - Chapter 8
A. Definition
B. Characteristics
C. Structure
1. Simple Enzymes
2. Conjugated Enzymes
D. Nomenclature of Enzymes
E. Classification of Enzymes
F. Enzyme--Substrate Reactions
G. Levels of Structure of Enzymes
H. Conditions Affecting Enzyme Activity
I. Location and Regularity of Enzyme Action
1. Exoenzymes
2. Endoenzymes
3. Constitutive Enzymes
4. Inducible Enzymes
J. Regulation of Enzymatic Activity
1. Competitive vs. noncompetitive inhibition
2. Reversible vs. irreversible
3. Energy Link Control
4. Feedback Control
5. Precursor Activation
6. General Processes
K. Regulation of Enzyme Synthesis
1. Constitutive Enzymes
2. Inducible Enzymes
3. Induction – Chapter 9.3
4. Repression – Chapter 9.3
II.
Metabolism - Chapter 8
A. Introduction
1. Definition
2. Catabolism
3. Anabolism
B. Coupling of Cellular Reaction
1. Free Energy Change
2. Calories
3. Exergonic Reactions
4. Endergonic Reactions
C. Oxidation and Energy Production
1. Oxidation
2. Reduction
D. Biochemical Pathways for Glucose Catabolism
1. Aerobic Respiration
Page 1
2. Anaerobic Respiration
3. Fermentation
E. Catabolism of Other Macromolecules
1. Catabolism of Lipids
2. Catabolism of Proteins
F. Photosynthesis
Page 2
BIO 2924
Mr. Smith
Unit III
Enzymes
An enzyme is an organic catalytic agent produced by a living cell.
A catalyst is a substance that has the capacity to speed up chemical reactions without itself being altered after the reaction is complete.
Characteristics of Enzymes
1. Most enzymes are proteins or proteins combined with other chemical groups, and other enzymes (ribozymes) are composed of RNA.
Since enzymes are proteins or RNA molecules,
a. their specific three-dimensional structure is essential to functionality.
b. they are often denatured by heat and changes in pH.
c. they cannot cross the plasma membrane via simple diffusion.
2.