Crayfish Heart Rate Lab
Introduction
Crayfish (Procambarus clarkia) are crustaceans that are related to lobsters, crabs, and shrimps (UNT Lab Manual, 2017). They normally inhabit fresh to partly salty water and feed on fish, carrion, plants, and detritus. Because they are completely aquatic, they rely on their gills for breathing, but can spend short periods of time out of the water if their gills will not dry. Like most crustaceans, they have a hard, calcified exoskeleton.
The heart is located in the upper portion of the cephalothorax, which is located just in front of the first abdominal segment (UNT Lab Manual, 2017). We can measure the heart rate of the crayfish by measuring the change of the impedance of the circuit that is created by placing fine wires on …show more content…
Because of this when they are faced with low water levels, they will actively seek a new source of. This activity will increase their metabolism, which then increases their heart rate (UNT Lab Manual, 2017).
In this experiment, three neurotransmitters are important: serotonin, acetylcholine, and epinephrine. Serotonin regulates the heart rate in invertebrates and will increase it, but in vertebrates it has variable effects (UNT Lab Manual, 2017). Acetylcholine will also speed up the crayfish’s heart rate, but in vertebrates will slow it down (UNT Lab Manual, 2017). Epinephrine will increase the heart rate in both invertebrates and vertebrates (UNT Lab Manual, 2017)
My hypothesis is that the decrease of the crayfish’s water should also be shown to be the slowest heart rate. While forcing the crayfish into an agitated state will increase the heart rate. All of the neurotransmitters will also increase the heart rate. While the relaxed state should serve as a baseline for the other exercises. …show more content…
We then transferred one ml of the solution into the container using a micropipettor with a blue tip. We then calculated the final concentration of the neurotransmitter in the water. We allowed the crayfish to sit under the foil, which we have placed back on the top of the container, for five minutes so that the serotonin would equilibrate between the water and the crayfish’s hemolymph. We then recorded the rate for five minutes. After that, we stopped recording and replaced the water with fresh saline. We used the same process for acetylcholine and
Crayfish (Procambarus clarkia) are crustaceans that are related to lobsters, crabs, and shrimps (UNT Lab Manual, 2017). They normally inhabit fresh to partly salty water and feed on fish, carrion, plants, and detritus. Because they are completely aquatic, they rely on their gills for breathing, but can spend short periods of time out of the water if their gills will not dry. Like most crustaceans, they have a hard, calcified exoskeleton.
The heart is located in the upper portion of the cephalothorax, which is located just in front of the first abdominal segment (UNT Lab Manual, 2017). We can measure the heart rate of the crayfish by measuring the change of the impedance of the circuit that is created by placing fine wires on …show more content…
Because of this when they are faced with low water levels, they will actively seek a new source of. This activity will increase their metabolism, which then increases their heart rate (UNT Lab Manual, 2017).
In this experiment, three neurotransmitters are important: serotonin, acetylcholine, and epinephrine. Serotonin regulates the heart rate in invertebrates and will increase it, but in vertebrates it has variable effects (UNT Lab Manual, 2017). Acetylcholine will also speed up the crayfish’s heart rate, but in vertebrates will slow it down (UNT Lab Manual, 2017). Epinephrine will increase the heart rate in both invertebrates and vertebrates (UNT Lab Manual, 2017)
My hypothesis is that the decrease of the crayfish’s water should also be shown to be the slowest heart rate. While forcing the crayfish into an agitated state will increase the heart rate. All of the neurotransmitters will also increase the heart rate. While the relaxed state should serve as a baseline for the other exercises. …show more content…
We then transferred one ml of the solution into the container using a micropipettor with a blue tip. We then calculated the final concentration of the neurotransmitter in the water. We allowed the crayfish to sit under the foil, which we have placed back on the top of the container, for five minutes so that the serotonin would equilibrate between the water and the crayfish’s hemolymph. We then recorded the rate for five minutes. After that, we stopped recording and replaced the water with fresh saline. We used the same process for acetylcholine and