Cromataghraphy
Dr.Tawfeq al.Qaimare
Rifqa Shawer
Lab report 5
21-3-2013
Adsorption Chromatography
Introduction:
The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube, it's a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column, giving this region a nearly dark blue color. However, when the flow of solution is discontinued and pure solvent is allowed to flow through the column, the process known as development occurs. The greenish dye is held tenaciously at the top of the column while the yellowish begins to separate and move down the column. As the development continues, the separation becomes more pronounced until there results a pure green zone in the upper part of the column and a pure yellow zone in the lower part of the column-as shown in the picture below-
This pattern called a CHROMATOGRAPHY.
This simple experiment illustrates all of the essential steps of a process which is in constant use in laboratories to effect separate mixtures of compounds, both organic and inorganic. It should be not inferred from the experiment that just mixtures with color can be separated; also the uncolored mixtures can be separated by this technique, but as might be expected, the details of the operation need to be modified, depending on nature of the substances being handled.
Objectives: To separate Organic compounds with the help of Column Chromatographic technique, another valuable applications are: 1. purification of compounds by removal of small amounts of contaminants. 2 .determination of the homogeneity of chemical substances. 3. comparison of compounds thought to be identical. 4. concentration of material from dilute solutions.
Table of physical constants:
Structure
M.F
M.W
M.P
Rifqa Shawer
Lab report 5
21-3-2013
Adsorption Chromatography
Introduction:
The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube, it's a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column, giving this region a nearly dark blue color. However, when the flow of solution is discontinued and pure solvent is allowed to flow through the column, the process known as development occurs. The greenish dye is held tenaciously at the top of the column while the yellowish begins to separate and move down the column. As the development continues, the separation becomes more pronounced until there results a pure green zone in the upper part of the column and a pure yellow zone in the lower part of the column-as shown in the picture below-
This pattern called a CHROMATOGRAPHY.
This simple experiment illustrates all of the essential steps of a process which is in constant use in laboratories to effect separate mixtures of compounds, both organic and inorganic. It should be not inferred from the experiment that just mixtures with color can be separated; also the uncolored mixtures can be separated by this technique, but as might be expected, the details of the operation need to be modified, depending on nature of the substances being handled.
Objectives: To separate Organic compounds with the help of Column Chromatographic technique, another valuable applications are: 1. purification of compounds by removal of small amounts of contaminants. 2 .determination of the homogeneity of chemical substances. 3. comparison of compounds thought to be identical. 4. concentration of material from dilute solutions.
Table of physical constants:
Structure
M.F
M.W
M.P