Describe The Difference Between 2-Apb And Orai2
We examined whether 2-APB and YM-58483 reduced calcium entry through store-operated calcium entry channels.
2-APB (Fig. 7C) and YM-58483 (Fig. 7E) similarly decreased store-operated calcium entry, as determined using the recalcification protocol. In the presence of 2-APB or YM-58483, readdition of 500 uM extracellular calcium in the recalcification protocol increased cytosolic calcium to 340 nm-to-380 nm ratios of ~2; in contrast, in the absence of pharmacological inhibition, readdition of 500 uM extracellular calcium increased cytosolic calcium to 340 nm-to-380 nm ratios of ~4
(Fig. 2B). Unlike the effect observed with cytosolic sodium,
Orai1 expression was not a determinant of whether 2-APB and
YM-58483 reduced calcium influx. Thus, Orai1
2-APB (Fig. 7C) and YM-58483 (Fig. 7E) similarly decreased store-operated calcium entry, as determined using the recalcification protocol. In the presence of 2-APB or YM-58483, readdition of 500 uM extracellular calcium in the recalcification protocol increased cytosolic calcium to 340 nm-to-380 nm ratios of ~2; in contrast, in the absence of pharmacological inhibition, readdition of 500 uM extracellular calcium increased cytosolic calcium to 340 nm-to-380 nm ratios of ~4
(Fig. 2B). Unlike the effect observed with cytosolic sodium,
Orai1 expression was not a determinant of whether 2-APB and
YM-58483 reduced calcium influx. Thus, Orai1