DIOSCOREA HISPIDA
BRINE SHRIMP LETHALITY ASSAY OF BAAY (Dioscorea hispida) LEAVES, STEMS, AND TUBERS
Rhea Cris Castillo, Ivan Dano, Eugene Manulat, Kc Reyna,
Elaine Sansait, and Arnel Tatoy
Graduate Studies, Philippine Normal University- Mindanao Campus, Prosperidad, Agusan del Sur, Philippines
Abstract
The present study was conducted to determine the lethal concentrations of the different parts of Dioscorea hispida through Brine Shrimp Lethality Assay. Cytotoxicity was evaluated in terms of lethal concentration. Each part of the plant was powdered and 0.1 gram of it was added to the first of the five wells. One (1) mL of the mixture was added to the vials with different concentrations. Fifteen brine shrimp nauplii were delivered into each vial and after 24 hours, the surviving brine shrimp were counted and the lethal concentrations were assessed. Results showed that all parts of Dioscorea hispida (leaves, stems, and tubers) are all toxic with lethal concentrations of 28.28 g/ml, 218.21 g/ml, and 9.19 g/ml respectively.
Keywords: Dioscorea hispida, lethal concentration, cytotoxicity testing, Brine shrimp Lethality Assay
INTRODUCTION
In times of food shortage, people find food alternatives to satisfy their everyday needs. Some people, especially the natives, would sometimes resort to root crops in lieu of rice as a primary source of energy, an example of which are the yams of the genus Dioscorea. Yam is the leading form of staple food for millions of people in the tropic and subtropical countries. Many yams are of economic importance as tuberous food crops. This tuber contains the plant food reserves, mainly starch, and it is often incorporated in the human diet (Sheikh, et.al., 2013). The underground tubers and the aerial tubers of both wild and cultivated varieties are harvested as a starch source in West Africa, parts of Southeast Asia, the Pacific Islands, India, and Central America (Webster, et.al., 1984). It contains reasonably substantial amount of protein,
Rhea Cris Castillo, Ivan Dano, Eugene Manulat, Kc Reyna,
Elaine Sansait, and Arnel Tatoy
Graduate Studies, Philippine Normal University- Mindanao Campus, Prosperidad, Agusan del Sur, Philippines
Abstract
The present study was conducted to determine the lethal concentrations of the different parts of Dioscorea hispida through Brine Shrimp Lethality Assay. Cytotoxicity was evaluated in terms of lethal concentration. Each part of the plant was powdered and 0.1 gram of it was added to the first of the five wells. One (1) mL of the mixture was added to the vials with different concentrations. Fifteen brine shrimp nauplii were delivered into each vial and after 24 hours, the surviving brine shrimp were counted and the lethal concentrations were assessed. Results showed that all parts of Dioscorea hispida (leaves, stems, and tubers) are all toxic with lethal concentrations of 28.28 g/ml, 218.21 g/ml, and 9.19 g/ml respectively.
Keywords: Dioscorea hispida, lethal concentration, cytotoxicity testing, Brine shrimp Lethality Assay
INTRODUCTION
In times of food shortage, people find food alternatives to satisfy their everyday needs. Some people, especially the natives, would sometimes resort to root crops in lieu of rice as a primary source of energy, an example of which are the yams of the genus Dioscorea. Yam is the leading form of staple food for millions of people in the tropic and subtropical countries. Many yams are of economic importance as tuberous food crops. This tuber contains the plant food reserves, mainly starch, and it is often incorporated in the human diet (Sheikh, et.al., 2013). The underground tubers and the aerial tubers of both wild and cultivated varieties are harvested as a starch source in West Africa, parts of Southeast Asia, the Pacific Islands, India, and Central America (Webster, et.al., 1984). It contains reasonably substantial amount of protein,
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