disinfectant and antibiotics effect on bacteria method
Method
1. Sterilise all of your equipment with ethanol, blue flame or disinfectant if you can flame it.
2. Collect all equipment together ensuring you collect your goggles and gloves as you are using a flame and gloves and goggles are part of safety precautions. (don’t wear gloves with messing with Bunsen burner only goggles, but use gloves when messing with chemicals ensuring goggles on all the time)
3. Clean your surface with disinfectant to sterilise your lab work surface, this will prevent contamination but leave the disinfectant to stand up to 4 minutes so it can penetrate the harder capsules of some bacteria.
4. Using a sterile pipette with draw some of your bacteria culture from the bottle making sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate.
5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate, making sure the lid of the culture container does not touch the surface, and that the lid of the agar plate is quickly replaced after this step has been carried out.
6. Sterilise the grabbing part and up the handle of the forceps, do this by wiping it with ethanol (let the ethanol dry before flaming it) and flaming the forceps, this will ensure they are sterile and won’t contaminate the experiment.
7. Take a disc (filter paper absorbed in disinfectant or antibiotics) and using the sterilised forceps to place the disc onto the seeded agar plate.
8. Gently press down onto the agar plate making sure to not creating any slices or disrupting the agar jelly
9. Seal the agar plate with two pieces of tape just at the sides making sure it can still breath other with the bacteria could turn anaerobic then label you plate so it can identified including the date it was made, the type of bacteria it is and place it in to the incubator.
10. There will be a set time and
1. Sterilise all of your equipment with ethanol, blue flame or disinfectant if you can flame it.
2. Collect all equipment together ensuring you collect your goggles and gloves as you are using a flame and gloves and goggles are part of safety precautions. (don’t wear gloves with messing with Bunsen burner only goggles, but use gloves when messing with chemicals ensuring goggles on all the time)
3. Clean your surface with disinfectant to sterilise your lab work surface, this will prevent contamination but leave the disinfectant to stand up to 4 minutes so it can penetrate the harder capsules of some bacteria.
4. Using a sterile pipette with draw some of your bacteria culture from the bottle making sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate.
5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate, making sure the lid of the culture container does not touch the surface, and that the lid of the agar plate is quickly replaced after this step has been carried out.
6. Sterilise the grabbing part and up the handle of the forceps, do this by wiping it with ethanol (let the ethanol dry before flaming it) and flaming the forceps, this will ensure they are sterile and won’t contaminate the experiment.
7. Take a disc (filter paper absorbed in disinfectant or antibiotics) and using the sterilised forceps to place the disc onto the seeded agar plate.
8. Gently press down onto the agar plate making sure to not creating any slices or disrupting the agar jelly
9. Seal the agar plate with two pieces of tape just at the sides making sure it can still breath other with the bacteria could turn anaerobic then label you plate so it can identified including the date it was made, the type of bacteria it is and place it in to the incubator.
10. There will be a set time and