Section 1 Lab Safety
1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts)
Biological Safety Cabinet, Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt)
Keep your workspace free of all unnecessary materials
3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt)
Avoid loose fitting items of clothing, Wear appropriate shoes sandals are not allowed
4. There are several safety tips to protect one’s mucous membranes and broken skin. Identify one of the recommended tips. (1 pt)
Do not apply makeup, put in contact lenses, or otherwise perform activities that might introduce lab organisms to your personal space.
5. When working in a microbiology lab, it is important to contain spills and discard materials properly. Describe how a spill should be cleaned up safely in a microbiology lab. (3 pts)
1. Wear a lab coat, disposable gloves, safety glasses or a face shield, and if needed, approved respiratory equipment.
2. Soak a paper towel(s) in an appropriate disinfectant (70% ethanol or fresh 10% bleach solution) and place around the spill area.
3. Working from the outer edges into the center, clean the spill area with fresh towels soaked in the disinfectant. Be sure to decontaminate any areas or surfaces that you suspect may have been affected by the spill. Allow 10 minutes contact time.
4. Place the paper towels and gloves into a biohazard bag and autoclave these materials to sterilize them.
5. Dispose of any contaminated clothing properly.
6. Wash your hands with a disinfectant soap.
6. Describe the procedures that one must do before leaving the lab. (3 pts)
Before leaving the lab, make sure all items have been returned to their appropriate location. After your work area is clear, wipe thoroughly with antimicrobial