DNA Extraction Lab Report
Ama, Djina, Frank, Kadijat, and Kharla
Ms. Bulahan
Writer:
Djina
Jan-18-15
Lab #6 - DNA Extraction lab
Introduction:
DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life, every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such insulin, antibiotics, and hormones. DNA can be extracted from many types of cells. For this lab, we extracted DNA from a strawberry and our own cheek cells. D
NA can be extracted from cells by a simple technique with household chemicals, enabling us to see strands of DNA with the naked eye. In this lab, we added a buffer solution composed of salt, clear dish detergent, and deionized water for procedure 1( the strawberry part). For procedure 2( our cheek cells), we used the same buffer solution but added red food coloring so we can distinguish the
DNA from the rest of the cellular components because it was all a white mixture. Finally, ethanol was added in the test tubes because DNA is soluble in water and alcohol causes
DNA to isolate from the solution, leaving behind all the cellular components that aren't soluble in alcohol. These solutions allow the breakdown of lipids surrounding the DNA such as the nuclear envelope and proteins that make up a cell membrane. A few minutes later, clumps of a white substance that looks like mucus can be seen. That mixture of DNA can be spooled with a stirring rod and pulled out of the solution.
Design for procedure 1:
Hypothesis –when DNA extraction buffer is added to a strawberry mixture
Ms. Bulahan
Writer:
Djina
Jan-18-15
Lab #6 - DNA Extraction lab
Introduction:
DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life, every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such insulin, antibiotics, and hormones. DNA can be extracted from many types of cells. For this lab, we extracted DNA from a strawberry and our own cheek cells. D
NA can be extracted from cells by a simple technique with household chemicals, enabling us to see strands of DNA with the naked eye. In this lab, we added a buffer solution composed of salt, clear dish detergent, and deionized water for procedure 1( the strawberry part). For procedure 2( our cheek cells), we used the same buffer solution but added red food coloring so we can distinguish the
DNA from the rest of the cellular components because it was all a white mixture. Finally, ethanol was added in the test tubes because DNA is soluble in water and alcohol causes
DNA to isolate from the solution, leaving behind all the cellular components that aren't soluble in alcohol. These solutions allow the breakdown of lipids surrounding the DNA such as the nuclear envelope and proteins that make up a cell membrane. A few minutes later, clumps of a white substance that looks like mucus can be seen. That mixture of DNA can be spooled with a stirring rod and pulled out of the solution.
Design for procedure 1:
Hypothesis –when DNA extraction buffer is added to a strawberry mixture