The amount of enzyme added (product
The amount of enzyme added (product
1. As shown in the graph above, the rate of activity increases as the enzyme concentration is increased. In summarization, this means that the more enzymes that are available, allow for more substrates to be converted into product within a smaller amount of time. However, the substrate becomes a limiting factor and when there are no more substrates to be broken down, the reaction rate becomes constant.…
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity.…
For Activity A, we first tested enzyme activity. First, we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then, we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that, we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that, we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting, we rinsed the unlabeled cup we used earlier when we tested enzyme activity. Then we used a H2O2 syringe to transfer 10 mL of H2O2 into the rinsed unlabeled cup. After five minutes, we transferred 1 mL of the boiling catalase into the unlabeled cup with H2O2 in it with an unused transfer pipet and observed the results. After testing the effect of boiling on enzyme activity, we tested for catalase in living tissue. First, we rinsed the unlabeled 60 mL cup we used earlier. Then, we used a scalpel to cut a small piece of liver. After that, we macerated the piece of liver with a glass rod. When the liver was macerated enough, we put it in a cup with 10 mL of H2O2, which was transferred into the cup with a H2O2 syringe. Lastly, we observed the cup.…
The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments.…
Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease.…
Therefore, we know that the rate and the extent of the reaction increases, (as measured by color change) due to greater enzyme activity.…
What is cellular work? Why must living things utilize energy with maximum efficiency? Define energy. Why do living things need a constant input of energy? Where does all energy in the biosphere originate? Define kinetic and potential energy. (Give biological examples). What are the two laws of thermodynamics? Give examples. What is entropy and what is the law of entropy? How do living things resist the law of entropy? Give biological examples. Define metabolism, catabolism and anabolism. What is free energy? What is an exergonic and endergonic reaction? What is G for these two reactions? What are the characteristics of each of these reactions? Define the ATP/ADP cycle. What is a coupled reaction? What does a coupled reaction allow? What can ATP be used for? What is a metabolic pathway? What are enzymes? What type of molecule are enzymes? What do enzymes do in a metabolic pathway? Where are enzymes of a specific metabolic pathway often located? What is a benefit of cells using metabolic pathways? What is the energy of activation. How are enzymes related to the energy of activation of a reaction? How do enzymes increase the rate of a chemical reaction? Show how enzymes work. What is a substrate? Why are enzymes specific for specific reactions? What is a degradation and a synthesis reaction? Explain the induced fit model of enzyme function. How are enzymes named? How does substrate concentration influence the rate of an enzyme reaction? What are two ways an enzyme can be regulated? What is enzyme inhibition, give an example. Explain how a metabolic pathway can be regulated. What are cofactors? What are coenzymes? What is oxidation? What is reduction? What is an oxidation/reduction reaction? How is hydrogen related to oxidation/reduction reactions? Explain how oxidation/reduction is related to photosynthesis and cellular respiration. Is glucose our only fuel for respiration?…
Design an experiment, based on the principles of diffusion and osmosis, that the assistant could…
Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won't denature in cold temperatures, but have slowed molecular activity. pH will also have an affect on an enzymes efficiency, when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable after the experiment was complete.…
The reaction goes faster initially. The relationship is linear. As the substrate concentration increase the initial reaction goes faster.…
(Click on the Save a Copy button on the panel above to save your report)…
We believe that if there is more substrate concentrated, then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets the chance to interact with, the higher the probability of a lot of the enzyme working, and the more enzyme working leads to the increase in enzyme activity.…
* Enzymes bind to substrates = this helps to “ensure” correct angle/orientation higher percentage of collisions will result in a reaction.…
How do variables temperature and surface area affect the rate of reaction occurring in the enzyme?…
Dixie earned her LPN and ADN from Hawkeye Community College. She then went on to complete her MSN at…