Preview

Dye Chromatography Lab

Good Essays
Open Document
Open Document
822 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Dye Chromatography Lab
In this experiment the objective is to separate dyes in washable markers to see how many different dyes are combined to create one color. The technique used to separate the dyes in this experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper, a tall clear glass, water, table salt, 1 tsp. measuring spoon, an 8 oz. measuring cup, pencil, ruler, 6 washable markers, and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown, teal, red, orange, black, and green. The salt solution served as the mobile phase while the chromatography paper served as the stationary phase. The first marker color tested is teal. The teal marker appears to contain one dye which is the actual color of the marker, teal. Although there is only one dye present in this color, the experiment shows that it has more affinity for the salt solution which is the mobile phase in this experiment because it traveled far from the starting point on the chromatography paper. This mainly states that the teal dye has more of an attraction to the solvent (mobile phase) than the paper (stationary phase). Therefore it moves more rapidly up the paper. …show more content…
The brown marker appears to have more than one dye present therefore it had to be mixed to create the color brown. The mixtures of dyes used to create this color are orange, pink, a pink hue, and a teal blue. The order from least to greatest in which they traveled from the starting point is orange, pink, pink hue, and teal blue. The orange dye has the greatest infinity for the paper or stationary phase because it traveled the least furthest from the starting point compared to the other dyes. The teal blue traveled the furthest from the starting point making it having the greatest affinity to the solvent or the mobile

You May Also Find These Documents Helpful

  • Good Essays

    Blue No. 5 Dye Analysis

    • 1002 Words
    • 5 Pages

    Explain the types of intermolecular interactions that would occur between the FD&C Red No.40, Blue No.1 and Yellow No. 5 food dyes and the paper.…

    • 1002 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Dye Lab for Chem 205

    • 2366 Words
    • 10 Pages

    The last means by which light could be generated is through chemiluminescence. In such a case a chemical reaction occurs, causing an excitation of the product. As this product decays into its standard state, light is emitted. This form of luminescent is different from the previous two in the sense that no absorption of light is required to induce the glow. The glow results from the product of the chemical reaction having to be in an exited state or of higher…

    • 2366 Words
    • 10 Pages
    Good Essays
  • Good Essays

    The purpose of this lab is to determine which color travels further up on the strip of paper due to the reaction of the different solvents (1% salt solution and alcohol).…

    • 815 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Food Dyes Lab

    • 545 Words
    • 3 Pages

    To complete this lab, I first mixed the solvent solution consisting of the proper ratio of water, salt, and isopropyl alcohol in a Pyrex measuring cup. I then prepared my chromatography papers for 2 trials by drawing, with a pencil, the appropriate lines and labels. Using a toothpick, I added the color to the paper, and then repeated until the color was strong. I repeated this step several more times for the M&Ms as the color solution was not as strong as the food dyes. After the dyes were dry, I rolled the paper and added it to the pie plate, which contained a small amount of the prepared solvent. I then waited for the solution to…

    • 545 Words
    • 3 Pages
    Powerful Essays
  • Good Essays

    Nt1310 Lab 1

    • 829 Words
    • 4 Pages

    Base on the order of movement of the dyes through the gel (yellow moved the farthest, then purple, then blue), students should have originally predicted that the orange G (yellow dye) was the smallest, then bromophenol blue (purple), then xylene cyanole (blue) was the largest. But based on the molecular weights above, the purple was actually bigger than the blue, yet it moved farther in the gel. View the image below.…

    • 829 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Food Dyes Lab

    • 671 Words
    • 3 Pages

    The initial step to determine the concentration of the dyes previously found was to prepare a set of serial dilutions at 20%/40%/60%/80%/100% dye concentration. To prepare the 20% dilution 1mL of red 40/blue 1 was combined with 4mL of DI water. This method was used to produce the remaining dilutions with 2mL/3mL, 3mL/2mL, 4mL/1mL, and 5mL/0mL (mL dye / mL DI water). Each of the previous dilutions was tested in the spectrometer and data was recorded. Using the data obtained two Beer Law plots were plotted for both dyes and trendlines were added. The slope of the trendline was found and used to calculate the actual (mol/L) and percent concentrations…

    • 671 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    We would use the color chart to determine the amount of color change. The problems we had during this experiment was that the pipette, beakers,…

    • 180 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    Hockey bruh

    • 482 Words
    • 4 Pages

    The purpose of this experiment is to observe the characteristic flame test colors of different .... Answers 1'0 Post-Lab Questions (Student answers will vary.) 1.…

    • 482 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    D.|HCl + blue dye|A5|The mixture also turned green instantly. Under white paper it is a nice emerald color and slightly darker under black paper. |…

    • 1353 Words
    • 6 Pages
    Good Essays
  • Good Essays

    ap english

    • 544 Words
    • 3 Pages

    The purpose of this lab is to test what colors are in certain colors, chromatology is used to separate the colors.…

    • 544 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Lab Rules

    • 45071 Words
    • 181 Pages

    Any chemical can be dangerous if it is misused. Always follow the instructions for the experiment. Pay close attention to the safety notes. Do not do anything differently unless told to…

    • 45071 Words
    • 181 Pages
    Good Essays
  • Good Essays

    Chromotography Lab

    • 3574 Words
    • 15 Pages

    Note: Please post this assignment to your dropbox. THE DROPBOX IS ANAGALOGOUS TO AN E-MAIL ACCOUNT. (To accomplish your assignment, you may cut&paste the observations section of the assignment into your text editor (such as Microsoft Word). Complete your answers - then send the answers as an attachment to the dropbox. Attachment examples include Word document, Excel graph, etc.) Your dropbox can be entered via the dropbox tab at the top of the screen. Make sure you title your assignments properly. You do not need to show your calculations. Edit the observations sheet to present the data in a clear fashion. USE CAPITAL LETTERS FOR THE TEXT/DATA YOU ENTER IN YOUR REPORT.…

    • 3574 Words
    • 15 Pages
    Good Essays
  • Good Essays

    Leroy

    • 304 Words
    • 2 Pages

    In this activity, five known dye samples and three unknown dye mixtures will be subjected to agarose gel…

    • 304 Words
    • 2 Pages
    Good Essays
  • Good Essays

    (As The Editors Of Encyclopædia Britannica say “Paper chromatography, technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper. The method consists of applying the test solution or sample as a spot near one corner of a sheet of filter paper. The paper is initially impregnated with some suitable solvent to create a stationary liquid phase. An edge of the paper close to the spot is then immersed in another solvent in which the components of the mixture are soluble in varying degrees. The solvent penetrates the paper by capillary action and, in passing over the sample spot, carries along with it the various components of the sample.”) they explain what and how paper chromatography is used for. They all have to do with colors. Having to do with finding the real dyes in colors. (As Science Buddies explain “What color is black ink? Sometimes things are not just what we think they are. Often things can be broken down into component parts. One way to find out it to use a common chemistry technique called chromatography.”) This quote explains that there can be many object in just one object, just like paper chromatography. The paper chromatography experiment it tested to see what kind of dyes are just in one…

    • 853 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Mebdt

    • 2756 Words
    • 12 Pages

    Manison, N. (2003). To dye or not to dye: Putting the blue dye test under the microscope.…

    • 2756 Words
    • 12 Pages
    Powerful Essays