Procedure :-
For the Lesson 4, which was carried out not on the same day as Lesson 3, the participants took the same roles as in the Lesson 3 Lab session. The subject, who was the 23 years old female, was made to relax after briefing her with the procedures of this study and connecting the electrodes similar to the previous experiment carried out, using the same software, materials and apparatus and the same precautions were taken into account under the same conditions.This part of the experiment would last for 50 seconds of recording and about 4-5 minutes of overall rest.
Here, as well, after the calibration is done for the 8 seconds, a relatively flat line is obtained, which indicates that further recording can be carried out. Having the subject in a supine condition for about 2 minutes with her eyes closed, the recording begins by clicking on ‘record’ and ‘suspend’ after 10 seconds. The director comes with a math problem and recorder clicks ‘resume’. Still, with the eyes closed, the subject is asked to solve the math problem, mentally and this would take approximately 20 seconds and the recorder clicks ‘suspend’. For the hyperventilation condition, the subject was asked to breathe heavily and faster for about 2 minutes maintaining the eyes-closed. The subject was also told that she could stop before 2 minutes if she felt dizzy and light headed. Only then, when she is recovering from hyperventilation, all relaxed, the recorder will click ‘resume’ for 10 seconds then ‘suspend’. Immediately, after this section, the subject was asked to open her eyes and the recorder will click on ‘resume’, still in a relaxed condition trying not to blink, for a further 10 seconds and ‘suspend’ consequently. Then all the electrode cables pinch connectors were removed and the electrodes were peeled off and thrown as they are not