Empagliflozin Lab Report
INTRODUCTION
Empagliflozin is a potent and selective sodium-dependent glucose co-transporter-2 inhibitor. The chemical name of empagliflozin is (1S)-1, 5-anhydro-1-(4-chloro-3-{4-[(3S)-tetrahydrofuran-3-yloxy] benzyl} phenyl)-D-glucitol and its molecular formula is C17H19ClO2Si.figure no 1. Empagliflozin selectively inhibits SGLT-2 in the kidney, resulting in direct, insulin-independent, elimination of glucose by the kidney. The primary pharmacodynamics effect of empagliflozin on inhibition of glucose re-uptake in the kidneys was demonstrated in mice, rats and dogs. All species showed increased urine glucose concentrations after treatment and it has effects of reducing blood sugar and improving insulin secretion. Empagliflozin pharmacokinetics …show more content…
Appropriate dilutions were prepared to obtain 10 μg/ml solutions was scanned on a UV spectrophotometer in the wavelength range of 200-400 nm in 1.0 cm cell against solvent blank and the spectra was recorded. Spectrum was taken, and λmax was determined. Optical parameters of the developed methods are tabulated in table 1.
Assay procedure
A total of 20 tablets of Empagliflozin were opened and the contents were weighed and mixed. Accurately weighed and powdered. An aliquot of powder equivalent to the weight of 1 tablet was accurately weighed and transferred to the volumetric flask and was dissolved in 100 ml of methanol and made up to volume with same solvent. The solutions were filtered through a 0.45 μm nylon filter and sonicated for about 15 min and then volume made up with methanol. This solution was filtered to eliminate any insoluble matter. The filtrate was collected in a clean flask. Appropriate dilutions were made with methanol and measured solution (100 μg/ml) for both zero order at 275nm and first order derivative at 282 nm spectrophotometric
Empagliflozin is a potent and selective sodium-dependent glucose co-transporter-2 inhibitor. The chemical name of empagliflozin is (1S)-1, 5-anhydro-1-(4-chloro-3-{4-[(3S)-tetrahydrofuran-3-yloxy] benzyl} phenyl)-D-glucitol and its molecular formula is C17H19ClO2Si.figure no 1. Empagliflozin selectively inhibits SGLT-2 in the kidney, resulting in direct, insulin-independent, elimination of glucose by the kidney. The primary pharmacodynamics effect of empagliflozin on inhibition of glucose re-uptake in the kidneys was demonstrated in mice, rats and dogs. All species showed increased urine glucose concentrations after treatment and it has effects of reducing blood sugar and improving insulin secretion. Empagliflozin pharmacokinetics …show more content…
Appropriate dilutions were prepared to obtain 10 μg/ml solutions was scanned on a UV spectrophotometer in the wavelength range of 200-400 nm in 1.0 cm cell against solvent blank and the spectra was recorded. Spectrum was taken, and λmax was determined. Optical parameters of the developed methods are tabulated in table 1.
Assay procedure
A total of 20 tablets of Empagliflozin were opened and the contents were weighed and mixed. Accurately weighed and powdered. An aliquot of powder equivalent to the weight of 1 tablet was accurately weighed and transferred to the volumetric flask and was dissolved in 100 ml of methanol and made up to volume with same solvent. The solutions were filtered through a 0.45 μm nylon filter and sonicated for about 15 min and then volume made up with methanol. This solution was filtered to eliminate any insoluble matter. The filtrate was collected in a clean flask. Appropriate dilutions were made with methanol and measured solution (100 μg/ml) for both zero order at 275nm and first order derivative at 282 nm spectrophotometric