Preview

Enzyme Activity

Good Essays
Open Document
Open Document
1211 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Enzyme Activity
Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide
Abstract
We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages, and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available, the quicker the reaction will happen except in one test, which might mean that a balanced concentration of the two is most beneficial.
Introduction
The objectives of these experiments were to observe the effects of the enzyme-substrate relationships and to record our findings. Enzymes increase the rate of reactions by lowering the energy needed to activate the reaction (McNeil et al. 2013). Enzymes will work with substrates to produce reactions and products and they will bind together at an active site. They will only bond to with particular molecules and environmental factors can also affect their productivity. They are proteins, and proteins are made up of many amino acids (Brian et al. 2013). We used the enzyme catalase that occurs naturally in many organisms to study the qualitative and quantitative results of enzymatic activity. My hypothesis is that the findings in these experiments will show that the enzyme catalase will increase the rate of reaction with the substrate.
Methods
In Activity 1 Procedure A, we had four test tubes filled with different components. The table below shows each tube’s components. In each test tube, we added 5.0 mL 3% H2O2. We recorded initial observations and checked frequently for changes. Table 1. Tube # | Contents | 1 | 1 mL H2O | 2 | ¼ × ¼” potato cube | 3 | 1 mL Enz. | 4 | 1 mL Enz boiled for 5 minutes, then cooled |

In Activity 1 Procedure B, we prepared two more test tubes with different substrates. In each empty tube we put 1 mL of enzyme. To that, we added

You May Also Find These Documents Helpful

  • Better Essays

    Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…

    • 2040 Words
    • 9 Pages
    Better Essays
  • Better Essays

    Bio 155 Lab Report

    • 1344 Words
    • 9 Pages

    effect of enzyme concentration and substrate concentration on the reaction rate, we performed a serial…

    • 1344 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Lab 6 enzymes

    • 1000 Words
    • 5 Pages

    Purpose: The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity.…

    • 1000 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Enzyme

    • 585 Words
    • 3 Pages

    For Activity A, we first tested enzyme activity. First, we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then, we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that, we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that, we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting, we rinsed the unlabeled cup we used earlier when we tested enzyme activity. Then we used a H2O2 syringe to transfer 10 mL of H2O2 into the rinsed unlabeled cup. After five minutes, we transferred 1 mL of the boiling catalase into the unlabeled cup with H2O2 in it with an unused transfer pipet and observed the results. After testing the effect of boiling on enzyme activity, we tested for catalase in living tissue. First, we rinsed the unlabeled 60 mL cup we used earlier. Then, we used a scalpel to cut a small piece of liver. After that, we macerated the piece of liver with a glass rod. When the liver was macerated enough, we put it in a cup with 10 mL of H2O2, which was transferred into the cup with a H2O2 syringe. Lastly, we observed the cup.…

    • 585 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Ap Biology Enzyme

    • 425 Words
    • 2 Pages

    1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions, such as, different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with, 1.5 ml of H2O2, 1.5ml of H2O and different amounts of enzyme drops, to determine how much oxygen gas is produced during the reaction since the pressure of the test tube will get higher as more oxygen gas is accumulated during the reaction.…

    • 425 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Enzymes

    • 2259 Words
    • 10 Pages

    enzyme, as well as a discussion of how structure and function of enzymes are affected…

    • 2259 Words
    • 10 Pages
    Powerful Essays
  • Better Essays

    The manipulated variable was the type of anti-freeze used for each area of grass in the experiment.…

    • 930 Words
    • 4 Pages
    Better Essays
  • Good Essays

    * Fill the 50 cm3 measuring cylinder with water. Invert it over the trough of water, with the open end under the surface of the water in the bowl and with the end of the rubber tubing in the measuring cylinder. Clamp in place.…

    • 465 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Enzyme Catalysis Lab

    • 1096 Words
    • 5 Pages

    Enzyme catalysis was observed in order to analyze how changes in temperature, pH, enzyme concentration, and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly, decreases, and levels off or completely stops, and can be further affected by environmental factors, which play a crucial role in regulating enzymes and metabolic processes.…

    • 1096 Words
    • 5 Pages
    Good Essays
  • Good Essays

    In the first part of the enzyme lab, we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together, we observed and saw a change in the color of the substance. The darker and more brown the substance got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased about 10% a minute until it sort of equilibrated at 4 minutes and didn’t change to the fifth minute mark. If we were to change anything we did in the experiment, we would make our comparisons to the chart more precise. Overall we thought it was successful.…

    • 1927 Words
    • 8 Pages
    Good Essays
  • Better Essays

    Enzyme Formal Lab

    • 2542 Words
    • 11 Pages

    The purpose of this investigation is to figure out how temperature, pH, and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to occur, allowing vital processes such as digestion, ATP production, and DNA replication to occur efficiently. Using a Vernier Gas Pressure Sensor the rate of reaction of the enzyme catalase as it decomposed Hydrogen Peroxide in a test tube was measured and then results were put on the screen of a Macintosh computer using Logger Pro software and Vernier computer software. Several trials were then done with one factor, either temperature, pH, or enzyme concentration being altered, while the remaining two stayed constant. The rate of reaction of catalase should increase with enzyme concentration until a certain point until it reaches its limit. For temperature the rate of reaction of catalase should increase until the enzyme catalase denatures, and then after the rate should start to decrease. When exposed to different pH levels the rate of reaction of catalase should increase as the enzyme catalase reaches its optimal pH level, once it’s at that point the rate of reaction should then decrease as the pH level goes farther away from catalase’s optimal pH level. The data for the trials of pH and enzyme concentration support the expected hypotheses of how the reaction rate would be affected, however the data did not support the hypothesis made for the effects of temperature on an enzyme’s reaction rate due to divergent data from the expected results. (need this line or is conclusion and results of data and trends summarized with data supported hypotheses) ask if titel too long,,…

    • 2542 Words
    • 11 Pages
    Better Essays
  • Good Essays

    ENZYMES

    • 1134 Words
    • 5 Pages

    2. To the tube labelled W, 5mL of water were added and to S, the…

    • 1134 Words
    • 5 Pages
    Good Essays
  • Good Essays

    A living system controls its activity through enzymes. Enzymes are made from hundreds or even thousands of amino acids connected in a very unique and specific order. Almost all enzymes are proteins, except for ribozymes. The chain of amino acids then folds into a unique shape. That shape not only allows the enzyme to carry out specific chemical reactions but to act as a very efficient catalyst. The enzyme speeds that reaction up tremendously. Each enzyme reacts with one specific reactant called a substrate that will form its products. The purpose of the experiments is to determine the effects of substrate concentration, reaction time and enzyme concentration on the direction of an enzyme reaction.…

    • 3143 Words
    • 13 Pages
    Good Essays
  • Powerful Essays

    (iii) then, you dealt with two enzymes in the lab, get some information on that...what substrates are those enzymes specific to.... and talk about absolute and relative specificity...state whether the enzymes have absolute or relative specificity.…

    • 3104 Words
    • 13 Pages
    Powerful Essays
  • Satisfactory Essays

    expriment report

    • 373 Words
    • 2 Pages

    2. 94 mL of distilled water and 49 mL of methanol were pipetted into a beaker which was placed in a water bath. A tea bag containing 20 g ion exchanger was submerged into the solution.…

    • 373 Words
    • 2 Pages
    Satisfactory Essays