AP Biology enzyme cataylst lab report
Sang Kim
Enzyme Catalyst
Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.
Background: The four different activities to the enzyme catalyst lab have similar but different backgrounds. Activity A’s background is to investigate the specific reaction of the decomposition of hydrogen peroxide by the enzyme, catalase. Hydrogen Peroxide decomposes slowly into water and oxygen, and the addition of catalase lowers the activation energy of the reaction until it proceeds to a room temperature. The catalase will is unchanged and is usable for to catalyze the reaction of more hydrogen peroxide. The background of activities A, B, and C is to determine the concentration of H2O2 in a solution. To do this, the concentration of an unknown will be used, such as a solution of 0.1 N, which is normal. When titration is done, 5 mL of acid will go in a beaker. When pH indicator paper is added, the paper will turn yellow if it is an acid and green if the solution is neutral. This is the endpoint of titration. In the activities, 2% potassium permanganate will be used to titrate H2O2. When the H2O2 reacts with the potassium
Enzyme Catalyst
Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.
Background: The four different activities to the enzyme catalyst lab have similar but different backgrounds. Activity A’s background is to investigate the specific reaction of the decomposition of hydrogen peroxide by the enzyme, catalase. Hydrogen Peroxide decomposes slowly into water and oxygen, and the addition of catalase lowers the activation energy of the reaction until it proceeds to a room temperature. The catalase will is unchanged and is usable for to catalyze the reaction of more hydrogen peroxide. The background of activities A, B, and C is to determine the concentration of H2O2 in a solution. To do this, the concentration of an unknown will be used, such as a solution of 0.1 N, which is normal. When titration is done, 5 mL of acid will go in a beaker. When pH indicator paper is added, the paper will turn yellow if it is an acid and green if the solution is neutral. This is the endpoint of titration. In the activities, 2% potassium permanganate will be used to titrate H2O2. When the H2O2 reacts with the potassium