Enzyme Lab Report
Sterling Hayden
AP Biology
Mrs. Proehl
24 November 2014
I.
Title
Effect of Differing pH, Temperature, and Enzyme Concentration on Catalase Reaction Rate
II.
Introduction
Enzymes are used to increase the rate of specific reactions in the body. Catalase, a specific enzyme,
speeds the breakdown of hydrogen peroxide, a toxic chemical produced by cells in the body, into water and oxygen (Cain and others, 2010). The oxygen can be observed as bubbles coming from the reaction site.
Catalase is found in many living tissues of organisms, including chicken liver. The purpose of this experiment is to determine what changes in pH, temperature, and enzyme concentration have on the rate catalase works to break down hydrogen peroxide. If the pH, temperature, or enzyme concentration changes, then the reaction rate of catalase will either speed up or slow down. III.
Materials and Procedures
Materials needed include 1 molar HCl solution, 1 molar NaOH solution, 6 test tubes, measuring
pipette, 10ml graduated cylinder, 40 ml 3% hydrogen peroxide solution, straightedged razor blade, scissors, forceps, stirring rod, fresh liver, fresh apple, fresh potato, test tube holders, ice bath, warm water bath, and boiling water bath.
A
Place 2 ml of the 3% hydrogen peroxide solution into a clean test tube. Using forceps and scissors,
cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod.
Observe the bubbles. Assume this reaction is rated 4 on a scale of 05. This reaction is the control group for the experiment. The 05 scale based on bubbles is the measurement technique for each experiment. Pour off the liquid into a second test tube. This used liquid is the independent variable. Add more liver to this
liquid. Record the reaction rate. The reaction rate will be the dependent variable in each experiment. Add another 2 ml of hydrogen peroxide to the liver remaining in the first test tube. Record the
AP Biology
Mrs. Proehl
24 November 2014
I.
Title
Effect of Differing pH, Temperature, and Enzyme Concentration on Catalase Reaction Rate
II.
Introduction
Enzymes are used to increase the rate of specific reactions in the body. Catalase, a specific enzyme,
speeds the breakdown of hydrogen peroxide, a toxic chemical produced by cells in the body, into water and oxygen (Cain and others, 2010). The oxygen can be observed as bubbles coming from the reaction site.
Catalase is found in many living tissues of organisms, including chicken liver. The purpose of this experiment is to determine what changes in pH, temperature, and enzyme concentration have on the rate catalase works to break down hydrogen peroxide. If the pH, temperature, or enzyme concentration changes, then the reaction rate of catalase will either speed up or slow down. III.
Materials and Procedures
Materials needed include 1 molar HCl solution, 1 molar NaOH solution, 6 test tubes, measuring
pipette, 10ml graduated cylinder, 40 ml 3% hydrogen peroxide solution, straightedged razor blade, scissors, forceps, stirring rod, fresh liver, fresh apple, fresh potato, test tube holders, ice bath, warm water bath, and boiling water bath.
A
Place 2 ml of the 3% hydrogen peroxide solution into a clean test tube. Using forceps and scissors,
cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod.
Observe the bubbles. Assume this reaction is rated 4 on a scale of 05. This reaction is the control group for the experiment. The 05 scale based on bubbles is the measurement technique for each experiment. Pour off the liquid into a second test tube. This used liquid is the independent variable. Add more liver to this
liquid. Record the reaction rate. The reaction rate will be the dependent variable in each experiment. Add another 2 ml of hydrogen peroxide to the liver remaining in the first test tube. Record the