Enzyme Synthesis Lab Report
The objectives of this experiment were to investigate the activity of enzymes, components that influence the enzyme’s activity, identify an unknown phosphatase, influence of inhibitors, and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown phosphatase analyzed showed it was an acidic phosphatase. Ph 5 had the most enzyme activity and pH 9 presented 1/3 of enzyme activity, while pH of 11 had little activity. The Vmax of 0mM, 10mM, and 20mM showed the inhibitor was competitive. In the
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Enzymes, a class of proteins, are a catalyst that speed up all the chemical reactions within the body (Reece et al. 2010). Without enzymes, life would not exist. Enzyme kinetics is the study of these enzymatic chemical reactions. Acknowledgment of enzyme kinetics is important in enzyme analysis in order to both understand the basic enzymatic mechanism and choosing an approach for enzyme analysis. Enzymatic chemical reactions are used globally in clinical diagnostic testing, industrial reactions, and in biomedical analysis. Knowing the pH optimum is significant in testing enzyme activity. Tests on enzyme activity depend upon the enzymes analyzed in ideal circumstances. In addition to, many factors influence the rate at which a reaction takes place such as, pH, temperature, enzyme and substrate concentration, and the effects of different inhibitors. The pH of the cell affects the enzyme activity. Enzymes have an optimal pH level. How pH alters the activity of an enzyme is measured by using a spectrometer. Ph does not only change the shape of the enzyme but how efficient substrate binds to the enzyme (Reece et al. 2010). In order to analyze the outcome of accumulating the enzyme concentration upon the reaction rate, the substrate must be abundant. In this experiment, the pH optimum for an unknown phosphatase and the effect of an inhibitor on enzyme activity were observed.
This field of science is important in biological catalysts and antibiotics. Enzymes are produced within the body and affect the rate of reactions within cells. Each enzyme is subjected to one substrate that it catalyzes. An enzyme binds to a substrate to catalyze a substance (Reece et al. 2010). The structure of enzymes are folded into elaborated shapes that allow specific molecules to fit into its active
Enzymes, a class of proteins, are a catalyst that speed up all the chemical reactions within the body (Reece et al. 2010). Without enzymes, life would not exist. Enzyme kinetics is the study of these enzymatic chemical reactions. Acknowledgment of enzyme kinetics is important in enzyme analysis in order to both understand the basic enzymatic mechanism and choosing an approach for enzyme analysis. Enzymatic chemical reactions are used globally in clinical diagnostic testing, industrial reactions, and in biomedical analysis. Knowing the pH optimum is significant in testing enzyme activity. Tests on enzyme activity depend upon the enzymes analyzed in ideal circumstances. In addition to, many factors influence the rate at which a reaction takes place such as, pH, temperature, enzyme and substrate concentration, and the effects of different inhibitors. The pH of the cell affects the enzyme activity. Enzymes have an optimal pH level. How pH alters the activity of an enzyme is measured by using a spectrometer. Ph does not only change the shape of the enzyme but how efficient substrate binds to the enzyme (Reece et al. 2010). In order to analyze the outcome of accumulating the enzyme concentration upon the reaction rate, the substrate must be abundant. In this experiment, the pH optimum for an unknown phosphatase and the effect of an inhibitor on enzyme activity were observed.
This field of science is important in biological catalysts and antibiotics. Enzymes are produced within the body and affect the rate of reactions within cells. Each enzyme is subjected to one substrate that it catalyzes. An enzyme binds to a substrate to catalyze a substance (Reece et al. 2010). The structure of enzymes are folded into elaborated shapes that allow specific molecules to fit into its active