Experiment 6: Respiration-Fermentation in Yeast

Time(minute) | Tube 1 | Tube 2 | Tube 3 | Tube 4 | Tube 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0.01 | 0 | 0.04 | 0 | 2 | 0 | 0.02 | 0.01 | 0.07 | 0 | 3 | 0 | 0.03 | 0.02 | 0.11 | 0 | 4 | 0 | 0.05 | 0.03 | 0.15 | 0.01 | 5 | 0 | 0.07 | 0.04 | 0.19 | 0.02 | 6 | 0 | 0.09 | 0.05 | 0.23 | 0.02 | 7 | 0 | 0.10 | 0.06 | 0.27 | 0.03 | 8 | 0 | 0.12 | 0.07 | 0.31 | 0.03 | 9 | 0 | 0.14 | 0.08 | 0.35 | 0.04 | 10 | 0 | 0.15 | 0.09 | 0.39 | 0.05 | 11 | 0 | 0.16 | 0.10 | 0.43 | 0.05 | 12 | 0 | 0.17 | 0.10 | 0.47 | 0.05 | 13 | 0 | 0.18 | 0.11 | 0.51 | 0.06 | 14 | 0 | 0.21 | 0.11 | 0.55 | 0.06 | 15 | 0 | 0.22 | 0.12 | 0.59 | 0.07 | 16 | 0 | 0.24 | 0.13 | 0.63 | 0.07 | 17 | 0 | 0.26 | 0.14 | 0.67 | 0.07 | 18 | 0 | 0.28 | 0.15 | 0.71 | 0.08 | 19 | 0 | 0.29 | 0.16 | 0.73 | 0.09 | 20 | 0 | 0.3 | 0.17 | 0.75 | 0.10 | Rate of CO(2)Production (mL/hr) | 0 | 0.3 | 0.17 | 0.75 | 0.10 |

Title:
Respiration-Fermentation in Yeast

Aim:
To study the different of solution affect the gas forms during fermentation in yeast.

Procedure:
Refer to Lab manual

Result:

Questions:
1. Yes.
2. Sucrose.
3. When no oxygen the ATP will be produce.
4. Brewery.
5. Aerobic glucose and aerobic glucose oxygen.
6. CO2 and aerobic water.
7. CO2 and aerobic ethanol.

Conclusion:
The purpose of this experiment is to determine sucrose gas form during fermentation with yeast is the most CO2. Sucrose did better than glucose because glucose is the simplest sugar and was there more as a control. Sucrose will produce the most CO2 because it is a complex sugar than the most sugar. Thus, sucrose is a good substrate for fermentation.

Discussion:
The data cannot be 100% reliable since it is difficult to measure the exact amount of CO2. Because the gas forms during fermentation will at the middle of the pipette.
Possible Errors:
The wrong amount of solution affected the gas level (mL) the pipette not fill enough of yeast solution affected the gas level (mL)