Food Dye Additives Lab Report

Title
Testing Kool-Aid Mix for Food Dye Additives
Purpose
What food dye additives go into the food products we consume and how do these additives affect our perception of that food?
Hypothesis
If “Black Cherry” flavored Kool-Aid Mix appears red, then it will contain the food dye additive Red-40 that emits a red pigment.
Background
Food color not only affects the appearance of food, it also affects our perception of how food tastes. Tests have shown that consumers will choose colored drinks over uncolored drinks when that color corresponds with the flavor of the drink. Because of this, producers add color to their products to appeal to the public, thus making a larger profit on these more desirable products. The government controls the substances
Label the four microcentrifuge tubes with with the appropriate candy’s name
2. Label the four cups
Pour the solution containing the dissolved colored candy coating into the microcentrifuge tube

Agarose Gel Electrophoresis
Agarose gel electrophoresis system 1
Agarose gel 1
Power Supply 1
Electrophoresis buffer, 1x TAE 275 ml
Blue 1 reference dye 15 µl
Yellow 5 reference dye 15 µl
Yellow 6 reference dye 15 µl
Red 40 reference dye 15 µl
Dyes extracted from the candy 4 labeled tubes
2-20 microliter adjustable- volume micropipette 1
Marking Pen 1 Procedure

1. Pulse spin the microcentrifuge tubes in the