Student Number: 2009-33281 Date submitted: 07-08-13
Exercise 2
Formulation, Testing of Hypothesis, and Experimental Design
I. Objectives:
a. to define diffusion and demonstrate this process in gases b. to cite molecular weight and time as two factors affecting the rate of diffusion c. to formulate a hypothesis on the relationship of each of these factors on the rate of diffusion d. to conduct and experiment to determine the effects of the two factors on the rate of diffusion e. to compute the partial rate and average rate of diffusion f. to conclude on the relationships of molecular weight and time on the rate of diffusion
II. Materials:
A. Formulation of Hypothesis a. Apparatus - glass tube - cotton balls b. Substances - hydrochloric acid (HCl) - ammonium hydroxide (NH4OH)
B. Testing the Hypothesis Concerning the Rate of Diffusion a. Apparatus - petri dish of agar-water gel with three wells b. Substances - potassium permanganate (KMnO4) - potassium dichromate (K2CR2O7) - methylene blue
III. Procedure:
A. Formulation of Hypothesis 1. Fasten the glass tube to a ring stand. 2. Simultaneously moisten two cotton balls with hydrochloric acid and ammonium hydroxide. 3. Plug each end of the glass tube with the cotton balls simultaneously. 4. Watch the formation of white smoke inside the tube and mark its position. Measure the distance (in cm) of the cottons to the smoke and tabulate.
B. Testing the Hypothesis Concerning the Rate of Diffusion 1. Obtain a petri dish of agar-water gel with three wells, and label each well as potassium permanganate (KMnO4), potassium dichromate (K2CR2O7), and methylene blue. 2. Place a drop of each substance into the well and cover the petri dish. 3. Measure the diameter (in mm) of the colored area. Set as data for zero minute. 4. At three-minute intervals for thirty
References: 1. Article about Plant Physiology. Retrieved July 6, 2013 from http://employees.csbsju.edu/ssaupe/biol327/Lecture/water.htm