Frog Sciatic Nerve Lab Report

The purpose of this experiment is to examine the results of stimulation on a frog’s sciatic nerve by looking at its compound action potential, the conduction velocity, and by quantifying the total and absolute refractory period between the nerve at room and cold temperature.
An oscilloscope, preamplifier, and stimulator were used to stimulate the frog’s nerve located in the nerve chamber. Threshold voltage was determined by stimulating the nerve at increasing voltages until a compound action potential resulted. Absolute threshold was determined similarly by increasing the voltage until no further increase in amplitude was observed. Conduction velocity was determined by recording 2 separate readings from the nerve at 2 different distances in