Fruit Fly Lab Report
Introduction:
Drosophila melanogaster commonly known as the fruit fly is considered a model organism in the field of genetics because of its short life cycle of about 10 weeks and the ability of the fly to produce a relatively large number of offspring at 50-70 eggs per day upon female maturity. The physical size of the male and female Drosophila is approximately 2.5 to 3 mm respectively Drosophila allowing for minimal storage space in a laboratory setting. The intricate nervous system of the fruit fly has made them very vital to genetic research in nervous system disorders and cancer research (Klug, 12).
The goal of the Drosophila melanogaster lab was to breed homozygous wild-type Drosophila melanogaster with homozygous mutant Drosophila …show more content…
Four vials with sponge plugs were obtained and labeled with the group name, cross number, lab number, and date. 2. Each vial was prepared with a culture containing food prepared by adding 1 scoop of food, and equal amount of water, and a pinch of yeast and allowed to sit for several minutes. 3. Separate vials containing virgin female and male flies of the Oregon-R wild-type were obtained 4. The flies were anesthetized by dipping an absorbent wand into a bottle of Triethylamine (fly nap). 5. The excess Fly nap on the wand was blotted onto a paper towel. 6. The wand was then placed into the vial containing Oregon-R wild-type flies just below the sponge stopper for approximately 60 seconds. 7. The anesthetized flies were removed from Oregon-R vial and place onto a white piece of paper. 8. Using a dissecting microscope the male and female flies were separated according to the following guidelines of Genitalia Differences listed in table 1.
Males | Females | Genitalia Differences | Smaller 2.5 mm | Usually larger | Dark blunt abdomens | Lighter colored pointed abdomens | Last segment of abdomen is solid black | Abdomen is striped to the end | Sex combs-black bristles on upper joint of forelegs | No sex combs …show more content…
Six male and female adult flies from the F1 generation were placed into the new vials and identified according to the initial P1 crosses and labeled for F1 cross. 19. The failed cross 4 white mutant, yellow mutant dihybrid cross was replaced with a dihybrid cross from another lab group and the data for that cross is listed in table 3 as cross 4b.
Day 22: 20. Adult flies from the F1 generation were removed from the vials and the F2 generation remained in the culture medium.
Day 31: 21. The F2 generation was anesthetized and observed microscopically and the physical characteristics, number of occurrences, and sex of the flies were documented in table 4. 22. The F2 generation was not returned to
Drosophila melanogaster commonly known as the fruit fly is considered a model organism in the field of genetics because of its short life cycle of about 10 weeks and the ability of the fly to produce a relatively large number of offspring at 50-70 eggs per day upon female maturity. The physical size of the male and female Drosophila is approximately 2.5 to 3 mm respectively Drosophila allowing for minimal storage space in a laboratory setting. The intricate nervous system of the fruit fly has made them very vital to genetic research in nervous system disorders and cancer research (Klug, 12).
The goal of the Drosophila melanogaster lab was to breed homozygous wild-type Drosophila melanogaster with homozygous mutant Drosophila …show more content…
Four vials with sponge plugs were obtained and labeled with the group name, cross number, lab number, and date. 2. Each vial was prepared with a culture containing food prepared by adding 1 scoop of food, and equal amount of water, and a pinch of yeast and allowed to sit for several minutes. 3. Separate vials containing virgin female and male flies of the Oregon-R wild-type were obtained 4. The flies were anesthetized by dipping an absorbent wand into a bottle of Triethylamine (fly nap). 5. The excess Fly nap on the wand was blotted onto a paper towel. 6. The wand was then placed into the vial containing Oregon-R wild-type flies just below the sponge stopper for approximately 60 seconds. 7. The anesthetized flies were removed from Oregon-R vial and place onto a white piece of paper. 8. Using a dissecting microscope the male and female flies were separated according to the following guidelines of Genitalia Differences listed in table 1.
Males | Females | Genitalia Differences | Smaller 2.5 mm | Usually larger | Dark blunt abdomens | Lighter colored pointed abdomens | Last segment of abdomen is solid black | Abdomen is striped to the end | Sex combs-black bristles on upper joint of forelegs | No sex combs …show more content…
Six male and female adult flies from the F1 generation were placed into the new vials and identified according to the initial P1 crosses and labeled for F1 cross. 19. The failed cross 4 white mutant, yellow mutant dihybrid cross was replaced with a dihybrid cross from another lab group and the data for that cross is listed in table 3 as cross 4b.
Day 22: 20. Adult flies from the F1 generation were removed from the vials and the F2 generation remained in the culture medium.
Day 31: 21. The F2 generation was anesthetized and observed microscopically and the physical characteristics, number of occurrences, and sex of the flies were documented in table 4. 22. The F2 generation was not returned to