Fungus Used in Genetics: Sordaria
Erin Turner
Sordaria Outline
1) Introduction
a) Natural laboratory in Israel, known as the evolutionary canyon, provides idea location to observe the crossing over frequency in Sordaria
a.i) South facing slope is much more dry and harsh of a climate
a.ii) North facing slope is a more temperate climate
a.iii) Since these two drastically different climates are in close proximity to each other it creates an ideal study location
b) In this particular lab study we collected baseline data concerning cross-over frequency under controlled lab conditions
b.i) In order to understand the phenomenon of crossing over we must understand what happens in a controlled setting
b.ii) The data that we collect will be used for comparison throughout the experiment
c) Crossing over can result in three different types
c.i) 4:4, 2:4:2, 2:2:2:2
c.ii) Since the chromosomes can cross over in different ways it results in different arrangements of asci
d) Research Questions
d.i) How does exposure to environmental stresses in the controlled conditions change the crossover frequency in the population of sordaria
d.ii) What is the crossover frequency of spore color gene with centromere in organisms grown under optimal growth conditions?
d.iii) What is the map distance between spore color gene and centromere predicted by crossover frequency?
e) Procedure
e.i) Circular dishes were cut into four sections, the tan types were placed diagonally from each other and the wild types were placed diagonally from each other
e.ii) Cross over frequencies were examined under a microscope and the order of asci that occurred (2:2:2:2, 2:4:2, 4:4) were recorded
2) Methods
a) Set up
a.i) The crossing over was set up using four samples of two different types of Sordaria (tan and wild)
a.ii) All tools that were used were sanitized before and after use
a.iii) Small samples of each type were cut and placed in their designated areas on the agar
a.iv) Since they were placed diagonally there
Sordaria Outline
1) Introduction
a) Natural laboratory in Israel, known as the evolutionary canyon, provides idea location to observe the crossing over frequency in Sordaria
a.i) South facing slope is much more dry and harsh of a climate
a.ii) North facing slope is a more temperate climate
a.iii) Since these two drastically different climates are in close proximity to each other it creates an ideal study location
b) In this particular lab study we collected baseline data concerning cross-over frequency under controlled lab conditions
b.i) In order to understand the phenomenon of crossing over we must understand what happens in a controlled setting
b.ii) The data that we collect will be used for comparison throughout the experiment
c) Crossing over can result in three different types
c.i) 4:4, 2:4:2, 2:2:2:2
c.ii) Since the chromosomes can cross over in different ways it results in different arrangements of asci
d) Research Questions
d.i) How does exposure to environmental stresses in the controlled conditions change the crossover frequency in the population of sordaria
d.ii) What is the crossover frequency of spore color gene with centromere in organisms grown under optimal growth conditions?
d.iii) What is the map distance between spore color gene and centromere predicted by crossover frequency?
e) Procedure
e.i) Circular dishes were cut into four sections, the tan types were placed diagonally from each other and the wild types were placed diagonally from each other
e.ii) Cross over frequencies were examined under a microscope and the order of asci that occurred (2:2:2:2, 2:4:2, 4:4) were recorded
2) Methods
a) Set up
a.i) The crossing over was set up using four samples of two different types of Sordaria (tan and wild)
a.ii) All tools that were used were sanitized before and after use
a.iii) Small samples of each type were cut and placed in their designated areas on the agar
a.iv) Since they were placed diagonally there