Preview

Gal4-Gfp Enhancer Trap System

Good Essays
Open Document
Open Document
431 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Gal4-Gfp Enhancer Trap System
Dr. Jim Haseloff A plant biologist working at the Department of Plant Sciences, University of Cambridge. His scientific interests are focused on the engineering of plant morphogenesis, using microscopy, molecular genetics, computational and synthetic biology techniques. Prior to joining the Department of Plant Sciences, Jim served as group leader at MRC Laboratory of Molecular Biology in Cambridge and his group developed advanced imaging techniques and modified fluorescent proteins for efficient use in plants. Before this, Jim was a research fellow at Harvard Medical School, working on trans-splicing ribozymes.
He has also worked at the CSIRO Division of Plant Industry, Canberra, and developed methods for the design of the first synthetic RNA enzymes with novel substrate specificities. Jim is deeply involved with teaching Synthetic Biology at the University of Cambridge, and is very interested in its wider potential as a tool for engineering biological systems and underpinning sustainable technologies.
He was the one who adapted the GAL4/VP16-UAS system for the construction of enhancer trap lines in Arabidopsis. (Lovell, 2013)

GAL4-GFP enhancer trap system
It’s a system for GAL4 targeted gene expression. An enhancer trap vector bearing a modified GAL4-VP16 gene was inserted randomly into the Arabidopsis genome by Agro bacterium mediated transformation. Cell specific activation of the GAL4-VP16 gene by cellular enhancer results in activation of a linked GFP gene, allowing simple characterization of expression patterns. Targeted expression of another gene (X) can be induced by genetic crossing. (Haseloff)

Fig1. Shows the two T-DNA constructs.

Agrobacterium-mediated transformation

Agrobacterium-mediated transformation is used to transform plant cells with a plasmid containing the modified GAL4-VP16 and UAS-GFP genes. The modified GAL4-VP16 gene is positioned adjacent to the Tr border sequence



References: A.Lovell.(2013).Synthetic Biology Project.Woodrow Wilson International Center for Scholars. J. Haseloff.GAL4 System.University of Cambridge. Adapted from, http://www.plantsci.cam.ac.uk/Haseloff/assembly/page138/page138.html

You May Also Find These Documents Helpful

  • Powerful Essays

    Vliet, Kent A., Christine M. Stracey, Michael R. Cottam. 2004. A Lab Manual For Integrated Principles of Biology. Pearson Custom Publishing, Boston, MA.…

    • 1589 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Genetic transformation is one of the most important processes in biotechnology. Essentially, genetic transformation involves the process where a cell (in this lab, a bacterial cell) takes up foreign DNA from its surroundings and incorporates it into its own DNA. This gene transfer is accomplished with the aid of a plasmid, a…

    • 1330 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Pentz, Lundy. The Biolab book 2nd Edition. 1989. USA. The Johns Hopkins University Press. P105 – 107.…

    • 3513 Words
    • 15 Pages
    Powerful Essays
  • Good Essays

    Plasmids are small circular autonomously replicating pieces of DNA that can be found inside of a prokaryotic bacterial cell. By barrowing a cell’s polymerase they replicate their own DNA. They are easy to extract from the bacterial cells due to their size. Plasmids are helpful for cloning foreign genes because of their ability to express antibiotic resistance as well their ability to be modified to express proteins of interest. A pGLO plasmid contains genes for the green florescent protein (GFP) as well as the gene for ampicillin resistance known as beta-lactamase. It also contains a gene regulation system (operon) that has the ability to control expression of the GFP gene in transformed cells known as araC. The source of GFP is naturally founds within a…

    • 463 Words
    • 2 Pages
    Good Essays
  • Good Essays

    In this lab, we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid, which is relatively small, circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism, it is absorbed into the organism’s own genetic code. After this occurs, the newly introduced gene begins coding for proteins, giving the organism a new trait. This process of genetic transformation is often used in the field of biotechnology. For example, genes that code for proteins that protect against herbicide are often added to crops such as corn so that farmers can target…

    • 702 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Mogel, p13). In addition to this, the genetic engineering of plants has the possible opportunity to…

    • 465 Words
    • 1 Page
    Good Essays
  • Good Essays

    Ribonucleic acid (RNA) plays a part in the genetic engineering process because the RNA is a strand of nucleotides. The amount of research required to confirm the desired strand can be substantial, however once the RNA needed is identified, it can then be isolated, and replicated for further…

    • 1218 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Oshinskie, M. (200?). The Rest Of The Story Behind Genetic Engineering, [internet].http://online.sfsu.edu/%7Erone/GEessays/tokarinterview.htm [accessed 18 JUNE 2008.]…

    • 1189 Words
    • 5 Pages
    Powerful Essays
  • Better Essays

    Rnai

    • 3925 Words
    • 16 Pages

    RNAi is an endogenous cellular mechanism that is present in some organisms, including plants and worms. Biologists have learned to use the RNAi mechanism to their advantage. By deliberately introducing defined sequences of dsRNA identical to the sequence of a gene, biologists can observe the physiological consequences of “silencing” that gene. Silencing genes in this way is also called reverse genetics because one can knock down the function of particular genes in a targeted way without mutating the gene.…

    • 3925 Words
    • 16 Pages
    Better Essays
  • Good Essays

    The main goal of synthetic life is to recreate life from nonliving components. Synthetic biology attempts to create new biological molecules and even novel living species capable of carrying out a range of important medical and industrial functions. From manufacturing pharmaceuticals to detoxifying polluted land and water. In medicine, it offers prospects of using designer biological parts as a starting point for an entirely new class of therapies and diagnostic tools (Nature). One of the aims of synthetic biology is to understand the many interactions in living cells and by fabricating biological systems and understanding how they function. Since natural biological systems are so complex, scientists in this field start by making simple synthetic systems and then studying what factors affect that fabricated system. In this way, the "design" of future synthetic systems can be continually improved as well as gaining a deeper insight to the complex interactions within those biological systems. Thus, the idea is to understand the complex interactions in living systems by building and designing them from bottom to top. Originally, this was the aim of the field of systems biology, which aims to understand the complexity of living systems by taking all the biological interactions as a whole and then putting forth models in order to describe how they give rise to intricate…

    • 915 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Vliet, K.A. (ed.). 1996. A Laboratory Manual for Integrated Principles of Biology: Part One – BSC2010L. Ginn Press, Needham Heights, Massachusetts.…

    • 1926 Words
    • 8 Pages
    Better Essays
  • Better Essays

    Pglo Transformation

    • 3036 Words
    • 13 Pages

    Genetic transformation is also seen in organisms that are multicellular. However, the process is more difficult and in some cases can be particularly challenging. The multicellular nature of most plants introduces the complication of transforming each cell of the plant in order to fully integrate the new information (2). Often the approach taken in higher organisms, such as plants, involves transforming an individual plant cell and then regenerating it into a whole organism (2).…

    • 3036 Words
    • 13 Pages
    Better Essays
  • Good Essays

    GMO Info

    • 705 Words
    • 3 Pages

    Drought tolerance and salinity tolerance of genetically modified plants is improved so that they can survive in different environments. As the world population grows and more land is utilized for housing instead of food production, farmers will need to grow crops in locations previously unsuited for plant cultivation. Creating plants that can withstand long periods of drought or high salt content in soil and groundwater will help people to grow crops in formerly inhospitable places.…

    • 705 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Full Thesis

    • 5420 Words
    • 27 Pages

    Genetic variations are the basic tools in the hands of breeders to develop new cultivars with better traits, like tolerance against various environmental stresses, resistance against pest and diseases and improved yield and quality.…

    • 5420 Words
    • 27 Pages
    Better Essays