Abstract
The purpose of this experiment was to establish the different stages of mitosis which occur in the tip of a garlic root. The root tip which was used had already been pre-stained with an aceto-orecein stain. The stain made the root tips more visible so that under a light microscope it would be much easier to distinguish where the root tip was. While under the light microscope the objective was to be able to identify and distinguish each of the stages, so the magnification of the light microscope was set to x10 first to find the cells which were undergoing mitosis thereafter the magnification was increased to x40 so that each individual phase could be clearly seen when taking place. The phases which could be seen were interphase, metaphase and anaphase. Introduction
“The study comes under the heading of cytogenetics which is the visual characterisation of genetic material and the study of how that material reacts during the life of the cell”. This experiment was designed to observe and distinguish the different stages of mitosis which take place in the tip of a garlic root. In order for the …show more content…
This could have been due to the amount of stain which was on the root tip as this may have made it harder to look for and identify which stage was taking place. The root tip itself may have been damaged while it was being transferred onto the microscope slide. The results of this experiment were compared to others and it was found that other results consisted of prophase being identified as well as interphase, metaphase and anaphase. From the results we see that interphase is the most easily identified phase this is because interphase is classed as the resting phase for a cell. Metaphase and anaphase are not as easy to identify as the cell does not spend a lot of time in these phases and during anaphase the cell may go from early to late