GEL ELECTROPHORESIS Assignment
GEL ELECTROPHORESIS: Go to the following link and work through the virtual lab on the gel electrophoresis process, filling in this worksheet as you go. Submit this worksheet for this week’s lab assignment. http://learn.genetics.utah.edu/content/labs/gel/ 1. Scientists use ___gel___ _______electrophoresis_____ when they need to sort DNA strands according to length.
2. The gel is a ______filter___________ that sorts DNA strands.
3. Electrophoresis is how we push DNA strands through the gel filter. What is used to make the DNA move through the gel? Electrical current
4. What physical property allows the DNA strands to sort themselves? DNA sequencing
5. What must one do to the sorted DNA strands to make them visible? staining
6. The buffer is a salt water solution that will let the _electrical___ _____charges_____ flow through the gel.
7. When preparing our gel, we will place a comb into one end of the gel. After the gel solidifies we remove the comb, leaving empty ____wells_______ for the DNA samples.
8. Each time you load a new and different sample of DNA, what must one first do to the micropipetter?
Get loading buffer dye
9. When placing the gel in the electrophoresis chamber, the DNA wells must be closest to which end of the chamber? (Underline the correct answer.)
negative (black) positive (red)
10. When ready to run a gel, one must connect and turn on the power supply. How can one tell that the current is running?
You look into the gel box for the flowing bubbles
2. The gel is a ______filter___________ that sorts DNA strands.
3. Electrophoresis is how we push DNA strands through the gel filter. What is used to make the DNA move through the gel? Electrical current
4. What physical property allows the DNA strands to sort themselves? DNA sequencing
5. What must one do to the sorted DNA strands to make them visible? staining
6. The buffer is a salt water solution that will let the _electrical___ _____charges_____ flow through the gel.
7. When preparing our gel, we will place a comb into one end of the gel. After the gel solidifies we remove the comb, leaving empty ____wells_______ for the DNA samples.
8. Each time you load a new and different sample of DNA, what must one first do to the micropipetter?
Get loading buffer dye
9. When placing the gel in the electrophoresis chamber, the DNA wells must be closest to which end of the chamber? (Underline the correct answer.)
negative (black) positive (red)
10. When ready to run a gel, one must connect and turn on the power supply. How can one tell that the current is running?
You look into the gel box for the flowing bubbles