Gel Electrophoresis Lab Report

Gel Electrophoresis is used to separate the haemoglobin component of blood.
Because each type of haemoglobin (HbA, HbS, Hbc and more) have different electrical charges, they will separate after undergoing gel electrophoresis. Firstly, a blood sample from the patient is taken and is applied to a cellulose acetate membrane strip that has been soaked in a buffer solution along with saponin. The red blood cells are lysed by the saponin while being soaked, therefore they release their haemoglobin. A charge is then applied at either end of the gel, and the haemoglobin bands move towards the cathode. The different types of haemoglobin can move away from one another at a different speed or rate, and the abnormalities can be discovered based on bands