Genetics Cross
Abstract
The main purpose of this experiment was to find which chromosome our unknown gene mutation is presented on and the exact location on that chromosome. In order to do so many cycles of crosses were completed using linkage analysis on Drosophila melanogaster, a type of fruit fly used in this experiment to identify our unknown gene mutation, unknown. Using virgin bar females crossed with Curly/Plum; Dichaete/Stubble male fruit flies represented our Discriminant Cross one (DC1). The DC1 piloted that the unknown mutation is sex linked dominant. Knowing the mutation is sex linked gives evidence that it is located on chromosome 1. Discriminant Cross two (DC2) is then performed basically to execute a check for DC1 by crossing a wild type female with a Plum/Stubble male fruit fly. In our case if the cross was done correctly all the females should carry the uknown mutation and none of the male progeny should show the unknown phenotype. Following DC2, another cross, called Mapping cross one (MC1), is then executed using a virgin unknown female with males carrying the dominant marker genes for the phenotypes yellow (y), crossveinless (cv), vermillion (v), and forked (f). The final cross that was performed was the Mapping cross two (MC2). The MC2 was carried out by crossing an F1 female from MC1 with an F1 male from MC1. The final step of the procedure is to validate the data obtained in order to confirm our results. This is done using the Chi-square analysis test. In conclusion, by using the data obtained from MC1 and MC2, unknown is approximated to be located at 45.57 m.u. According to the book of mutations in the lab the exact location for unknown is at 57 m.u. Unknown is then found to be bar (b).
Introduction
Drosophila melanogaster is most frequently used due to its small size, a conveniently short life cycle of about two weeks, and a small enough genome that helps us understand new mutations compared to our own genome. The goal in
The main purpose of this experiment was to find which chromosome our unknown gene mutation is presented on and the exact location on that chromosome. In order to do so many cycles of crosses were completed using linkage analysis on Drosophila melanogaster, a type of fruit fly used in this experiment to identify our unknown gene mutation, unknown. Using virgin bar females crossed with Curly/Plum; Dichaete/Stubble male fruit flies represented our Discriminant Cross one (DC1). The DC1 piloted that the unknown mutation is sex linked dominant. Knowing the mutation is sex linked gives evidence that it is located on chromosome 1. Discriminant Cross two (DC2) is then performed basically to execute a check for DC1 by crossing a wild type female with a Plum/Stubble male fruit fly. In our case if the cross was done correctly all the females should carry the uknown mutation and none of the male progeny should show the unknown phenotype. Following DC2, another cross, called Mapping cross one (MC1), is then executed using a virgin unknown female with males carrying the dominant marker genes for the phenotypes yellow (y), crossveinless (cv), vermillion (v), and forked (f). The final cross that was performed was the Mapping cross two (MC2). The MC2 was carried out by crossing an F1 female from MC1 with an F1 male from MC1. The final step of the procedure is to validate the data obtained in order to confirm our results. This is done using the Chi-square analysis test. In conclusion, by using the data obtained from MC1 and MC2, unknown is approximated to be located at 45.57 m.u. According to the book of mutations in the lab the exact location for unknown is at 57 m.u. Unknown is then found to be bar (b).
Introduction
Drosophila melanogaster is most frequently used due to its small size, a conveniently short life cycle of about two weeks, and a small enough genome that helps us understand new mutations compared to our own genome. The goal in
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