Gram Stain Lab Report

When I received my slant culture, I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides, I made and stained, my results were ambiguous. Without putting the slide into the microscope, I noticed one was pink and the other purple. To resolve it, I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started the protocol for the lactose test2 which would help me determine which test to conduct next to narrow down list of possible bacteria. I conducted the protocol for MR test3 and citrate test4 the same day as the lactose test to speed up the process of elimination. With conducting the MR. Citrate and Lactose test all in the same day, I learned that time management was a big deal and I learned quick and caught up with the class. The next class I observed my lactose tube and my results were AG, yellow with bubbles. The
K.phumoniae is not airborne but this bacterium is easily spread in hospitals and it can be caught by anyone who comes into contact with the infected person’s feces if proper precautions are not taken. It is recommended that if anyone needed to enter a room with a patient that has a K.phumoniae related illness, to wear a gown and gloves. As for the patient, it is recommended to wash their hands profusely to prevent any chance of anyone else contracting the illness. Some, not all K.Phumoniae are highly resistant to antibiotics. K.Phumoniae that produces an enzyme called carbapenems it doesn’t allow the antibiotics to destroy the bacteria and treat the patient and to be able to determine which antibiotics will work has to be determined by the doctor. The K.Phumoniae that is found in the gut can be treated with